ALLATOSTATIC NEUROPEPTIDES FROM THE COCKROACH BLATTELLA-GERMANICA (L) (DICTYOPTERA, BLATTELLIDAE) - IDENTIFICATION, IMMUNOLOCALIZATION AND ACTIVITY
ALLATOSTATIC NEUROPEPTIDES FROM THE COCKROACH BLATTELLA-GERMANICA (L) (DICTYOPTERA, BLATTELLIDAE) - IDENTIFICATION, IMMUNOLOCALIZATION AND ACTIVITY
复制标题
DOI：
10.1016/0167-0115(94)90172-4
复制
发表时间：
1994-10-21
影响因子：
--
通讯作者：
THORPE, A
中科院分区：
文献类型：
--
作者：
BELLES, X;MAESTRO, JL;THORPE, A
从德国小蠊脑提取物中分离得到四种抑制异速生长的神经肽。这些肽的一级结构指定为Leu-Tyr-Asp-Phe-Gly-Leu-NH 2（BLAST-1）、Asp-Arg-Leu-Tyr-Ser-Phe-Gly-Leu-NH 2（BLAST-2）、Ala-Gly-Ser-Asp-Gly-Arg-Leu-Tyr-Ser-Phe-Gly-Leu-NH 2（BLAST-3）和Ala-Pro-Ser-Ser-Ala-Gln-Arg-Leu-Tyr-Gly-Phe-Gly-Leu-NH 2（BLAST-4）。每个肽显示C-末端氨基酸序列的相似性，蟑螂allatostatin和丽蝇callatostatin。这四种肽在体外抑制B的未交配雌性的咽侧体产生保幼激素。德国的。在外侧神经分泌神经元和通往咽侧体的轴突通路中观察到对allatostatin的免疫反应性。
Four allatostatic neuropeptides were isolated from extracts of the brain of the cockroach Blattella germanica. The primary structures of these peptides were assigned as Leu-Tyr-Asp-Phe-Gly-Leu-NH2 (BLAST-1), Asp-Arg-Leu-Tyr-Ser-Phe-Gly-Leu-NH2 (BLAST-2), Ala-Gly-Ser-Asp-Gly-Arg-Leu-Tyr-Ser-Phe-Gly-Leu-NH2 (BLAST-3) and Ala-Pro-Ser-Ser-Ala-Gln-Arg-Leu-Tyr-Gly-Phe-Gly-Leu-NH2 (BLAST-4). Each of the peptides showed C-terminal amino acid sequence similarity to cockroach allatostatins and blowfly callatostatins. The four peptides inhibited in vitro juvenile hormone production by corpora allata from virgin females of B. germanica. Immunoreactivity against allatostatins was seen in the lateral neurosecretory neurons and in the axonal pathway leading to the corpora allata.