The E3 ubiquitin ligase HECTD3 regulates ubiquitination and degradation of Tara
The E3 ubiquitin ligase HECTD3 regulates ubiquitination and degradation of Tara
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E3泛素连接酶HECTD3调节Tara的泛素化和降解
DOI：
10.1016/j.bbrc.2008.01.022
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发表时间：
2008-03-21
影响因子：
3.1
通讯作者：
Huang, He
中科院分区：
文献类型：
--
作者：
Yu, Jian;Lan, Jianping;Huang, He
塔拉被鉴定为鸟嘌呤核苷酸交换因子Trio和TRF I的相互作用伴侣。塔拉被认为参与了许多重要的基本细胞过程，从肌动蛋白重塑，定向细胞运动，细胞周期调控。然而，其确切作用需要进一步阐明。在这里，我们确定了一种新的塔拉结合蛋白HECTD 3，HECT E3泛素连接酶的推定成员。HECTD 3在体外直接结合塔拉，在体内与塔拉形成复合物。HECTD 3的过表达增强了体内塔拉的泛素化，并促进了塔拉的周转，而小干扰RNA对HECTD 3的消耗减少了塔拉的降解。此外，HECTD3的缺失导致多极纺锤体形成。这些结果表明HECTD 3可能通过调节塔拉的泛素化和降解来促进细胞周期进程。(c)2008年爱思唯尔公司All rights reserved.
Tara was identified as an interacting partner of guanine nucleotide exchange factor Trio and TRF I. Tara is proposed to be involved in many important fundamental cellular processes, ranging from actin remodeling, directed cell movement, to cell cycle regulation. Yet, its exact roles required further elucidation. Here, we identify a novel Tara-binding protein HECTD3, a putative member of HECT E3 ubiquitin ligases. HECTD3 directly binds Tara in vitro and forms a complex with Tara in vivo. Overexpression of HECTD3 enhances the ubiquitination of Tara in vivo and promotes the turnover of Tara, whereas depletion of HECTD3 by small interfering RNA decreases Tara degradation. Furthermore, depletion of HECTD3 leads to multipolar spindle formation. All these findings suggest that HECTD3 may facilitate cell cycle progression via regulating ubiquitination and degradation of Tara. (c) 2008 Elsevier Inc. All rights reserved.