Cloning of 17beta-hydroxysteroid dehydrogenase-I cDNAs from Japanese eel ovary.
Cloning of 17beta-hydroxysteroid dehydrogenase-I cDNAs from Japanese eel ovary.
复制标题
从日本鳗鱼卵巢中克隆 17β-羟基类固醇脱氢酶-I cDNA。
DOI：
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发表时间：
2000
期刊：
影响因子：
--
通讯作者：
K. Yamauchi
中科院分区：
文献类型：
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作者：
Y. Kazeto;S. Ijiri;Hajime Matsubara;S. Adachi;K. Yamauchi
17β-羟基类固醇脱氢酶-I (17β-HSD-I) 是产生雌二醇-17β (E(2)) 的关键类固醇生成酶。编码17β-HSD-I的cDNA首次在低等脊椎动物中从硬骨鱼日本鳗鱼的卵巢中克隆出来。从这些 cDNA 推导的氨基酸序列与哺乳动物 17β-HSD-Is 大约有 50% 相同。通过 Northern 印迹法在卵黄发生前的卵巢中未检测到 17β-HSD-I mRNA。然而，在通过促性腺激素处理人工成熟的鱼中获得的早期卵黄卵巢中，转录本丰度有所增加，但此后似乎没有进一步变化。在人肾293细胞中表达的重组17β-HSD-I选择性地将雌酮转化为E(2)，但雄烯二酮、睾酮或E(2)没有转化为任何其他类固醇。尽管人们广泛认为E(2)是由其他硬骨鱼物种中的睾酮产生的，但鳗鱼17β-HSD-I的底物特异性表明日本鳗鱼卵巢中存在通过雌酮从雄烯二酮产生E(2)的类固醇生成途径。
17beta-hydroxysteroid dehydrogenase-I (17beta-HSD-I) is a key steroidogenic enzyme for estradiol-17beta (E(2)) production. cDNAs encoding 17beta-HSD-I were cloned for the first time in lower vertebrates from the ovary of a teleost, the Japanese eel. The deduced amino acid sequence from these cDNAs was approximately 50% identical to mammalian 17beta-HSD-Is. 17beta-HSD-I mRNA was not detected in previtellogenic ovaries by Northern blotting. However, transcript abundance increased in early vitellogenic ovaries obtained from fish artificially matured by gonadotropic treatment, but thereafter did not appear to change further. Recombinant 17beta-HSD-I expressed in human kidney 293 cells selectively converted estrone to E(2), but androstenedione, testosterone, or E(2) were not converted to any other steroids. Although it is widely accepted that E(2) is produced from testosterone in other species of teleosts, the substrate specificity of eel 17beta-HSD-I suggests that a steroidogenic pathway for production of E(2) from androstenedione via estrone exists in the Japanese eel ovary.
人胎盘雌二醇 17 β-脱氢酶：催化区域中带有组氨酸的肽的序列。
影响因子：
2.9
作者：
Murdock,GL;Chin,CC;Warren,JC
通讯作者：
Warren,JC
DOI：
10.1016/0006-291x(92)92373-6
发表时间：
1992-10-15
影响因子：
3.1
作者：
OBEID, J;WHITE, PC
通讯作者：
WHITE, PC