What Controls the "Off/On Switch" in the Toehold-Mediated Strand Displacement Reaction on DNA Conjugated Gold Nanoparticles?
What Controls the "Off/On Switch" in the Toehold-Mediated Strand Displacement Reaction on DNA Conjugated Gold Nanoparticles?
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DNA 缀合金纳米颗粒上的立足点介导的链置换反应中的“关/开”开关是由什么控制的？
DOI：
10.1021/acs.langmuir.5b01671
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发表时间：
2015
期刊：
影响因子：
3.9
通讯作者：
Liang Haojun
中科院分区：
文献类型：
--
作者：
Yao Dongbao;Wang Bei;Xiao Shiyan;Song Tingjie;Huang Fujian;Liang Haojun
在DNA动力学纳米技术中，立足点介导的DNA链置换反应已经证明了其构建复杂自治系统的能力。在大多数情况下，反应在纯DNA溶液中进行，该溶液基本上是单相系统。在本工作中，我们系统地研究了在非均相介质中的反应，其中实施置换作用的链在金纳米颗粒上缀合。通过监测由支点介导的链置换反应驱动的球形核酸（SNA）组装的动力学，我们观察到显著差异，即，当入侵的立足点从7个碱基扩展到8个碱基时，反应速率中的“关/开开关”行为突然跳跃。这些现象归因于由与AuNP缀合的高密度侵入链产生的空间位阻效应。在此基础上，研制了具有良好选择性的INHIBIT逻辑门。
In DNA dynamic nanotechnology, a toehold-mediated DNA strand-displacement reaction has demonstrated its capability in building complex autonomous system. In most cases, the reaction is performed in pure DNA solution that is essentially a one-phase system. In the present work, we systematically investigated the reaction in a heterogeneous media, in which the strand that implements a displacing action is conjugated on gold nanoparticles. By monitoring the kinetics of spherical nucleic acid (SNA) assembly driven by toehold-mediated strand displacement reaction, we observed significant differences, i.e., the abrupt jump in behavior of an "off/on switch", in the reaction rate when the invading toehold was extended to eight bases from seven bases. These phenomena are attributed to the effect of steric hindrance arising from the high density of invading strand conjugated to AuNPs. Based on these studies, an INHIBIT logic gate presenting good selectivity was developed.