Micro RNA-19a interferes with IL-10 expression in peripheral dendritic cells of patients with nasal polyposis.
Micro RNA-19a interferes with IL-10 expression in peripheral dendritic cells of patients with nasal polyposis.
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Micro RNA-19a 干扰鼻息肉患者外周树突状细胞中 IL-10 的表达。
DOI：
10.18632/oncotarget.16555
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发表时间：
2017-07-25
期刊：
影响因子：
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通讯作者：
Yang PC
中科院分区：
文献类型：
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作者：
Luo XQ;Shao JB;Xie RD;Zeng L;Li XX;Qiu SQ;Geng XR;Yang LT;Li LJ;Liu DB;Liu ZG;Yang PC
鼻息肉的发病机制有待进一步研究。微小RNA（miR）在过敏性炎症的发展中起作用。产生白细胞介素（IL）-10的树突状细胞（DC）具有免疫耐受性。本研究验证了miR-17-92簇与外周DC中抑制IL-10相关的假设。本研究收集了26例鼻息肉患者的外周血标本。从血液样品中分离CD 11 c DC并分析IL-10的表达。我们观察到，与健康受试者相比，息肉患者外周DC中的IL-10表达显著降低。息肉组DC中miR-19 a的表达水平明显高于正常对照组，而miR-17-92簇的其余5个成员的表达水平则无明显差异。暴露于重组IL-4抑制DC中IL-10的表达，通过阻断组蛋白去乙酰化酶-11或敲低DC中的miR-19 a基因来消除。结论miR-19 a在抑制外周DC中IL-10的表达中起重要作用，可能成为鼻息肉免疫治疗的靶点。
The pathogenesis of nasal polyp is to be further investigated. Micro RNA (miR) plays a role in the development of allergic inflammation. Interleukin (IL)-10-producing dendritic cells (DC) have immune tolerogenic properties. This study test a hypothesis that miR-17-92 cluster is associated with suppressing IL-10 in peripheral DC. In this study, peripheral blood samples were obtained from 26 patients with nasal polyp. The CD11c DCs were isolated from the blood samples and analyzed for the expression of IL-10. We observed that, as compared with healthy subjects, the IL-10 expression in peripheral DC was significantly lower in polyp patients. The levels of miR-19a, but not the rest 5 members of the miR-17-92 cluster, were markedly higher in DCs in polyp group. Exposure to recombinant IL-4 suppressed the IL-10 expression in DCs, which was abolished by blocking histone deacetylase-11 or knocking down the miR-19a gene in DCs. We conclude that miR-19a plays a critical role in the suppression of IL-10 in peripheral DCs, which may be a target in the immune therapy for nasal polyp.