Serum levels of interleukin-32 and interleukin-6 in granulomatosis with polyangiitis and microscopic polyangiitis: association with clinical and biochemical findings
Serum levels of interleukin-32 and interleukin-6 in granulomatosis with polyangiitis and microscopic polyangiitis: association with clinical and biochemical findings
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DOI：
10.1684/ecn.2019.0439
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发表时间：
2019-12-01
影响因子：
2.8
通讯作者：
Morawski, Krzysztof
中科院分区：
文献类型：
--
作者：
(Wojciechowska), Joanna Krajewska;Koscielska-Kasprzak, Katarzyna;Morawski, Krzysztof
背景资料：抗神经元胞浆抗体（ANCA）相关性血管炎是一种病因不明的自身免疫性疾病，伴有细胞因子水平失调。目的：本研究的主要目的是评估抗神经细胞胞浆抗体（ANCA）相关性血管炎、肉芽肿性多血管炎（GPA）、显微镜下多血管炎（MPA）血清水平、促炎细胞因子白细胞介素（IL）-32和IL-6血清水平之间的临床相关性。方法：研究对象为71例患者，其中GPA组47例，MPA组24例。分析所有患者的血清IL-32和IL-6浓度，并与10名对照组的水平进行比较。分别使用DuoSet和Quantikine HS ELISA评价IL-32和IL-6。IL-32和IL-6浓度与疾病相关的临床和实验室检查结果相关。结果：GPA组和MPA组IL-32和IL-6水平均显著高于对照组，尤以GPA组IL-32水平升高最为明显。GPA组IL-32水平与抗蛋白酶3 - ANCA（PR 3-ANCA）水平呈正相关（P < 0.0001），MPA组IL-32水平与抗髓过氧化物酶ANCA（MPO-ANCA）水平呈正相关（P = 0.049）。IL-32水平与GPA和MPA的疾病活动度呈正相关（P < 0.0001）。GPA患者肺部，皮肤和肌肉骨骼受累的IL-6血清水平最高。MPA患者的皮肤表现与IL-6水平呈正相关（P = 0.05）。ANCA阳性GPA患者IL-6水平显著升高（P = 0.036）。ANCA阳性和ANCA阴性患者之间IL-32值无显着差异。结论：GPA和MPA患者血清IL-32和IL-6水平高于对照组。IL-32水平与疾病活动呈正相关。
Background: Antineutrophil cytoplasmic antibody (ANCA)-associated vasculitis is an autoimmune disorder of unknown etiology with dysregulated cytokines levels. Objectives: The main aim of this study was to assess the clinical correlation between antineutrophil cytoplasmic antibody (ANCA)-associated vasculitis, granulomatosis with polyangiitis (GPA) serum levels of the microscopic polyangiitis (MPA), serum levels of the proinflammatory cytokines, interleukin (IL)-32 and interleukin-6. Methods: Study included 71 patients, 47 with GPA and 24 with MPA. Serum IL-32 and IL-6 concentrations were analyzed in all patients, and compared with levels observed in 10 controls. IL-32 and IL-6 were evaluated using DuoSet and Quantikine HS ELISA, respectively. IL-32 and IL-6 concentrations were correlated with disease-related clinical and laboratory findings. Results: IL-32 and IL-6 levels were significantly higher in GPA and MPA than in controls, especially IL-32 levels in GPA were elevated. IL-32 concentrations correlated positively with anti-proteinase 3 - ANCA (PR3-ANCA) levels in GPA (P < 0.0001), and with anti-myeloperoxidase ANCA (MPO-ANCA) in MPA(P = 0.049). IL-32 levels correlated positively with disease activity inGPA and MPA(P < 0.0001). GPA patients with pulmonary, cutaneous, and musculoskeletal involvement presented the highest IL-6 serum levels. Cutaneous manifestations correlated positively with IL-6 levels in MPA patients (P = 0.05). ANCA-positive patients with GPA expressed significantly high IL-6 levels (P = 0.036). No significant difference in IL-32 values was observed between ANCA-positiveandANCA-negativepatients. Conclusions: Patientswith GPAandMPApresenthigher serumIL-32 and IL-6 levels than controls. IL-32 levels correlate positively with disease activity.