Inclusion of proteins into calcium oxalate crystals precipitated from human urine: a highly selective phenomenon.
Inclusion of proteins into calcium oxalate crystals precipitated from human urine: a highly selective phenomenon.
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将蛋白质包含在从人尿中沉淀的草酸钙晶体中：一种高度选择性的现象。
DOI：
10.1093/clinchem/37.9.1589
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发表时间：
1991
影响因子：
9.3
通讯作者：
V. Marshall
中科院分区：
文献类型：
--
作者：
I. Doyle;R. Ryall;V. Marshall
草酸钙尿石基质中丰富的蛋白质引发了人们对其在结石形成中的作用的猜测。在这项研究中，我们想要表征与尿液中草酸钙结晶早期阶段相关的蛋白质的组成。在健康男性和女性的尿液中加入草酸钙负荷，可诱导草酸钙结晶。收获晶体，脱矿，分离剩余的蛋白质，用聚丙烯酰胺凝胶电泳法和Western blotting进行鉴定。大多数尿蛋白没有在晶体中检测到，或者只有少量存在。最丰富的尿液大分子，Tamm-Horsfall糖蛋白，在晶体提取物中明显缺失。与晶体相关的主要蛋白质，一种以前未知的尿液成分，我们称为晶体基质蛋白(CMP；分子质量，30,000 Da)，在来自女性尿液的晶体中更为普遍。我们得出结论，大多数尿蛋白在草酸钙晶体的形成中没有直接作用。然而，蛋白质CMP对草酸钙晶体表现出显著的亲和力，可能在结石的发病机制中起重要作用。
The abundance of protein in the matrix of calcium oxalate uroliths has fueled speculation regarding its role in stone genesis. In this study, we wanted to characterize the composition of the proteins associated with early stages of calcium oxalate crystallization in urine. Calcium oxalate crystallization was induced in urine from healthy men and women by the addition of an oxalate load. The crystals were harvested and demineralized, and the proteins remaining were separated and characterized by polyacrylamide gel electrophoresis and Western blotting. Most urinary proteins were not detected in the crystals or were present in only small quantities. The most abundant urinary macromolecule, Tamm-Horsfall glycoprotein, was notably absent from the crystal extracts. The predominant protein associated with the crystals, a previously unknown urinary constituent that we call crystal matrix protein (CMP; molecular mass, 30,000 Da), was more prevalent in the crystals derived from female urine. We conclude that most urinary proteins play no direct role in calcium oxalate crystal formation. However, the protein CMP exhibits a remarkable affinity for calcium oxalate crystals and may be important in stone pathogenesis.