Delivery of plasmid DNA to mammalian cells using polymer-gold nanorod assemblies.

Delivery of plasmid DNA to mammalian cells using polymer-gold nanorod assemblies.
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使用聚合物-金纳米棒组件将质粒 DNA 递送至哺乳动物细胞。

DOI:
10.1007/978-1-62703-336-7_9
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发表时间:
2013
期刊:
Methods in molecular biology (Clifton, N.J.)
影响因子:
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通讯作者:
Rege,Kaushal
Rege,Kaushal
中科院分区:
--
文献类型:
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作者:
Ramos,James;Huang,Huang-Chiao;Rege,Kaushal

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功能化和表面改性的金纳米棒(GNRs)已成为有前途的车辆提供几种治疗剂。易于功能化,增加的稳定性,生物相容性和尺寸依赖性等离子体特性使金纳米棒在传感,成像和递送到不同细胞类型方面具有吸引力。在这里,我们证明了使用聚电解质包被的GNRs(PE-GNRs)用于将质粒DNA递送到哺乳动物细胞进行转基因表达。
Functionalized and surface-modified gold nanorods (GNRs) have emerged as promising vehicles for the delivery of several therapeutic agents. Ease of functionalization, increased stability, biocompatibility, and size-dependent plasmonic properties make gold nanorods attractive in sensing, imaging, and delivery to different cellular types. Here, we demonstrate the use of polyelectrolyte-coated GNRs (PE-GNRs) for delivering plasmid DNA to mammalian cells for transgene expression.