Detection of stromal cells in peripheral blood progenitor cell collections from breast cancer patients
Detection of stromal cells in peripheral blood progenitor cell collections from breast cancer patients
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DOI：
10.1038/sj.bmt.1700890
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发表时间：
1997-08-01
影响因子：
4.8
通讯作者：
Minguell, JJ
中科院分区：
文献类型：
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作者：
Fernandez, M;Simon, V;Minguell, JJ
生长因子动员的外周血祖细胞（PBPC）移植被广泛应用于多种肿瘤性疾病的治疗，而在PBPC收获物中存在多种辅助免疫细胞和肿瘤细胞已被证实，基质细胞的存在尚未报道。在本研究中，我们研究了乳腺癌患者PBPC收获物中基质细胞的存在，从PBCP收获物中培养的低密度细胞产生含有成纤维细胞样和大帽状圆细胞的粘附层。这些细胞表达阳性免疫荧光染色的胶原蛋白I、胶原蛋白III、纤连蛋白、VCAM-1（CD 106）、ICAM-1（CD 54）和间充质抗原，其被单克隆抗体SH 2和SH 3识别。PBPC来源的基质细胞不表达抗原CD 34、CD 45和CD 14。在11/14例患者的PBPC收获物中检测到基质细胞（中值0.63%;范围0.02-2.32），并且它们的浓度与PBPC中的CD 34（+）细胞的数量相关。
Transplantation of growth factor-mobilized peripheral blood progenitor cells (PBPC) is widely used in the treatment of several neoplastic diseases, While in PBPC harvests the presence of several accessory immune and tumor cells has been documented, that of stromal cells has not been reported, In the present study, we investigated for the presence of stromal cells in growth factor-mobilized PBPC harvests from breast cancer patients, Low-density cells from PBCP harvests in culture gave rise to an adherent layer containing fibroblast-like and large hat round cells, These cells express positive immunofluorescence staining for collagen I, collagen III, fibronectin, VCAM-1 (CD106), ICAM-1 (CD54) and mesenchymal antigens recognized by monoclonal antibodies, SH2 and SH3. PBPC-derived stromal cells do not express antigens CD34, CD45 and CD14, Stromal cells were detected in the PBPC harvests of 11/14 patients (median 0.63 %; range 0.02-2.32) and their concentration correlates with the number of CD34(+) cells in PBPC.