Functional assessment of precursors from murine bone marrow suggests a sequence of early B lineage differentiation events
Functional assessment of precursors from murine bone marrow suggests a sequence of early B lineage differentiation events
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DOI：
10.1016/s1074-7613(00)80186-7
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发表时间：
2000-03-01
期刊：
影响因子：
32.4
通讯作者：
Kincade, PW
中科院分区：
文献类型：
--
作者：
Tudor, KSRS;Payne, KJ;Kincade, PW
来自鼠骨髓的大多数谱系标记阴性（Lin（-））TdT（+）细胞缺乏CD 34，但显示低密度的c-kit以及IL-7 R α和FIk-2/FIt-3受体。具有这些特征的单个细胞在培养中产生CD 45 RA（+）、CD 19（-）以及CD 19（+）淋巴细胞。CD 45 RA + CD 19-骨髓细胞分为三个不重叠的亚群。一个亚群缺乏DX 5和Ly-6C抗原，在培养中产生CD 19（+）细胞。进一步分析表明，大多数Lin（-）TdT（+）细胞和所有CD 45 R（+）CD 19（-）DX 5（-）Ly-6C（-）细胞上存在CD 24。Mac-1/CD 11 B b在这两个B谱系前体亚群中不存在，而IL-7 R α在随后分化为CD 19（+）和基质细胞非依赖性阶段中保留。这些发现与以前描述的B淋巴细胞前体形成对比，并提出了一系列早期分化事件。
Most lineage marker-negative (Lin(-))TdT(+) cells from murine marrow lack CD34 but display c-kit at low density as well as IL-7R alpha and FIk-2/FIt-3 receptors. Single cells with these characteristics generated CD45RA(+)CD19(-) as well as CD19(+) lymphocytes in culture. CD45RA+CD19- marrow cells were resolved into three nonoverlapping subsets. One subset, lacking DX5 and Ly-6C antigens, yielded CD19(+) cells in culture. Further analysis demonstrated CD24 on most Lin(-)TdT(+) cells and all CD45R(+)CD19(-)DX5(-)Ly-6C(-) cells. Mac-1/CD11b was absent from these two subsets of B lineage precursors, while IL-7R alpha was retained during subsequent differentiation to a CD19(+) and stromal cell-independent stage. These findings contrast with previous descriptions of B lymphocyte precursors and suggest a sequence of early differentiation events.