Thermal Shift Assay in Ferroptosis.
Thermal Shift Assay in Ferroptosis.
复制标题
铁死亡中的热位移测定。
DOI：
10.1007/978-1-0716-3433-2_16
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发表时间：
2023
期刊：
影响因子：
--
通讯作者：
Ghosh,Sayan
中科院分区：
文献类型：
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作者：
Bammidi,Sridhar;Hose,Stacey;Handa,JamesT;Sinha,Debasish;Ghosh,Sayan
铁凋亡是最近描述的细胞死亡过程，其依赖于不受调节的细胞铁积累和氧化应激的诱导。铁凋亡与几种人类疾病有关;因此，有必要进行旨在更好地了解途径和阐明未来药物开发途径的研究。目前靶向细胞中的铁凋亡/氧化应激的测定仅限于蛋白质印迹和成像技术，并且不幸的是仅提供了不足以有效评估新型药物（配体）的广泛理解。具体而言，这些测定不能提供关于配体与这些过程相关的特定蛋白质的相互作用的见解。在此，我们讨论了一种基于细胞的热位移试验，该试验能够在特定的细胞条件下筛选针对铁凋亡和/或氧化应激途径的配体。这些数据将为靶向这一途径的药物开发提供详细的初步证据。
Ferroptosis is a recently described process of cell death that is dependent on unregulated cellular iron accumulation with induction of oxidative stress. Ferroptosis has been linked to several human diseases; therefore, investigations aimed at better understanding the pathway and elucidating avenues for future drug development are warranted. Current assays that target ferroptosis/oxidative stress in cells is limited to western blotting and imaging techniques, and unfortunately provide only a broad understanding that is insufficient to effectively assess novel drugs (ligands). Specifically, these assays do not provide insights about ligand interactions with specific proteins associated with these processes. Herein, we discuss a cell-based thermal shift assay that enables screening of ligands under specific cellular conditions for targeting ferroptosis and/or oxidative stress pathways. These data would provide detailed preliminary evidence required for drug development that targets this pathway.