Rhipicephalus sanguineus (Ixodida, Ixodidae) as intermediate host of a canine neglected filarial species with dermal microfilariae
Rhipicephalus sanguineus (Ixodida, Ixodidae) as intermediate host of a canine neglected filarial species with dermal microfilariae
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DOI：
10.1016/j.vetpar.2011.07.031
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发表时间：
2012-02-10
影响因子：
2.6
通讯作者：
Bain, O.
中科院分区：
文献类型：
--
作者：
Brianti, E.;Otranto, D.;Bain, O.
出现真皮微丝虫病的狗的丝状线虫的生活史研究很少。继最近检索到的真皮微丝线虫鉴定为Cercopithifilaria sp.在一只来自西西里(意大利)的狗身上，这项研究旨在评估棕色狗的血色里皮头绦虫作为这种丝虫物种的中间宿主的作用。用300只血吸虫若虫对一只受感染的狗进行了实验性的蜱虫感染。分别于取食当天、取食后10d、20d、30d和50d的5个时间点采集吸血若虫，进行显微解剖和分子检测，以检测其体内丝虫的存在和发育阶段。共从狗身上采集到270只充血若虫和发育中的丝状幼虫，在200只扁虱中检出10只(5%)。剥离后30天，4只(2%)被解剖的硬壳中有3期幼虫感染。对181份样品进行分子检测，有12份(6.6%)检出脑红假单胞菌。本研究表明，血吸虫若虫取食一只自然感染天牛的狗。可以摄取微丝虫，微丝虫发育到第三个感染阶段，因此表明这种硬虱物种可能是这种鲜为人知的犬丝状动物的中间宿主。(C)2011爱思唯尔B.V.保留所有权利。
The life cycles of filarioids of dogs presenting dermal microfilariae have been little studied. Following the recent retrieval of dermal microfilariae identified as Cercopithifilaria sp. in a dog from Sicily (Italy), this study was designed to assess the role of the brown dog tick Rhipicephalus sanguineus as an intermediate host of this filarial species. An experimental tick infestation was performed on an infected dog using 300 nymphs of R. sanguineus. Engorged nymphs were collected and examined by both microscopic dissection and molecular analysis at five time points (i.e., the same day of tick detachment and 10, 20, 30 and 50 days post-detachment) to detect the presence and developmental stage of filariae in the ticks. A total of 270 engorged nymphs were collected from the dog and developing filarioid larvae detected in 10(5%) out of 200 ticks dissected. Infective third-stage larvae were observed in 4 (2%) of the all dissected ticks, 30 days post-detachment. Twelve (6.6%) out of 181 samples molecularly tested were positive for Cercopithifilaria sp. This study demonstrates that nymphs of R. sanguineus feeding on a dog naturally infected by Cercopithifilaria sp. can ingest microfilariae, which develop up to the third infective stage thus suggesting that this tick species might act as an intermediate host of this little known canine filarioid. (C) 2011 Elsevier B.V. All rights reserved.