Construction of Chinese hamster chromosome specific DNA libraries and their use in the analysis of spontaneous chromosome rearrangements in different cell lines.
Construction of Chinese hamster chromosome specific DNA libraries and their use in the analysis of spontaneous chromosome rearrangements in different cell lines.
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中国仓鼠染色体特异性DNA文库的构建及其在不同细胞系自发染色体重排分析中的应用。
DOI：
10.1159/000134000
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发表时间：
1995
期刊：
影响因子：
--
通讯作者：
A. Natarajan
中科院分区：
文献类型：
--
作者：
A. Balajee;I. Domínguez;A. Natarajan
中国仓鼠（Cricetulus griseus）及其衍生的各种细胞系已广泛用于放射生物学和遗传毒性研究。本文报道了用接头/接头法构建中国仓鼠1、2、3、5、6、7和8号染色体特异性DNA文库的方法。这些探针的特异性已通过FISH在原代胚胎成纤维细胞（CHE）中期染色体上检查。CHO不同细胞系的二倍体染色体数目通常在19 - 21之间变化，由于许多自发重排，核型分析非常困难。利用中国仓鼠染色体特异性DNA文库，我们分析了三种不同的中国仓鼠转化细胞系V79，CHO-KI和CHO-9的自发染色体重排。结果表明，在CHO-9和CHO-K1细胞中，第一对和第五对染色体中的每一对都参与相互易位。用染色体特异性探针2、3、5、6、7和8获得的杂交模式表明在V79、CHO-KI和CHO-9细胞中发生了涉及末端和间质易位的重排。
Chinese hamster (Cricetulus griseus) and a wide variety of cell lines derived from it have been extensively used for radiobiological and genotoxicity studies. In this report, we describe the development of chromosome-specific DNA libraries for Chinese hamster chromosomes 1, 2, 3, 5, 6, 7 and 8 by the linker/adaptor method. The specificity of these probes have been checked by FISH on primary embryonic fibroblast (CHE) metaphase chromosomes. The diploid number of chromosomes in different cell lines of CHO often varies from 19 to 21 and karyotyping is very difficult owing to many spontaneous rearrangements. Using Chinese hamster chromosome-specific DNA libraries, we have analysed the spontaneous chromosome rearrangements in three different Chinese hamster transformed cell lines, V79, CHO-KI and CHO-9. The results indicate that one of each of the first and fifth pair of chromosomes are involved in reciprocal translocations in both CHO-9 and CHO-K1 cells. The hybridisation patterns obtained with chromosome-specific probes 2, 3, 5, 6, 7 and 8 indicated rearrangements have occurred involving terminal and interstitial translocations in V79, CHO-KI and CHO-9 cells.