THE STRUCTURE AND FORMATION OF PROTEIN GRANULES IN THE FAT BODY OF AN INSECT.
THE STRUCTURE AND FORMATION OF PROTEIN GRANULES IN THE FAT BODY OF AN INSECT.
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DOI：
10.1083/jcb.26.3.857
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发表时间：
1965-09-01
期刊：
影响因子：
--
通讯作者：
Collins JV
中科院分区：
文献类型：
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作者：
Locke M;Collins JV
在蝴蝶Calpodes ethlius的幼虫中，脂肪体在化蛹前约30至35小时开始以颗粒形式储存蛋白质，此时内表皮正在被再吸收。至少可以区分两种颗粒。第一个出现的颗粒是高尔基复合体囊泡内的颗粒。这些可以通过在其表面处并入来自微泡的材料以及通过彼此聚结来增加尺寸。后来，在化蛹前约10小时，另一种颗粒的出现是由高尔基体囊泡衍生的成对膜内的内质网（ER）区域的分离引起的。这些ER隔离体中的几个合并，其外部隔离膜融合。然后，核糖体和膜可能消失，颗粒变得与高尔基体形成的蛋白质颗粒难以区分，或者核糖体可能保留并嵌入致密的晶体蛋白中，形成蛋白质和RNA的储存体。线粒体以与ER区域相同的方式分离在成对的膜内。分离的线粒体也以类似的方式合并。当内膜丢失时，一组隔离体的结构与细胞溶体的结构难以区分。如本文所述，成对膜内的隔离在分离大量溶解或大量隔离的区域中可能具有普遍的重要性。
In the larva of the butterfly Calpodes ethlius, the fat body begins to store protein in the form of granules at about 30 to 35 hours before pupation, at a time when the endocuticle is being resorbed. At least two sorts of granule can be distinguished. The first granules to arise are those within vesicles of the Golgi complex. These may increase in size by incorporating material from microvesicles at their surface and by coalescence with one another. Later, at about 10 hours before pupation, another sort of granule arises by the isolation of regions of the endoplasmic reticulum (ER) within paired membranes derived from Golgi vesicles. Several of these ER isolation bodies coalesce, with fusion of their outer isolating membranes. The ribosomes and membranes may then disappear and the granules become indistinguishable from the protein granules formed from Golgi vesicles, or the ribosomes may remain and be embedded in dense crystalline protein, forming a storage body for both protein and RNA. Mitochondria are isolated within paired membranes in the same way as regions of the ER. The isolated mitochondria also coalesce in a similar manner. When the inner membranes are lost, the structure of a group of isolation bodies is indistinguishable from that of a cytolysome. Isolation within paired membranes, as described here, may be of general importance in segregating regions of massive lysis or massive sequestration.