Against a whole-genome shotgun
Against a whole-genome shotgun
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DOI：
10.1101/gr.7.5.410
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发表时间：
1997-05-01
期刊：
影响因子：
7
通讯作者：
Green, P
中科院分区：
文献类型：
--
作者：
Green, P
人类基因组计划正进入决定性的最后阶段，基因组序列将在全球多个实验室的大规模努力中确定。一些测序小组正在扩大其吞吐量；在接下来的几年里，他们将需要达到每年接近0.5 gb的总容量，以便在2005年的目标日期之前完成3gb的基因组序列。目前，所有的研究小组都在使用一个克隆接一个克隆的方法，对已知染色体位置的细菌克隆（通常40 - 400kb大小）进行测序直至完成。除其他优点外，这允许独立探索各种替代测序策略和方法，而无需冗余的努力。尽管现在考虑实现一种不同的方法还不算太晚，但任何这样的方法都必须具有与当前方法一样高的成功概率，并提供显著的优势（例如降低成本）。我在这里认为，韦伯和迈尔斯提出的全基因组鸟枪法不满足这两个条件。
The human genome project is entering its decisive final phase, in which the genome sequence will be determined in large-scale efforts in multiple laboratories worldwide. A number of sequencing groups are in the process of scaling up their throughput; over the next few years they will need to attain a collective capacity approaching half a gigabase per year to complete the 3-Gb genome sequence by the target date of 2005. At present, all contributing groups are using a clone-by-clone approach, in which mapped bacterial clones (typically 40–400 kb in size) from known chromosomal locations are sequenced to completion. Among other advantages, this permits a variety of alternative sequencing strategies and methods to be explored independently without redundancy of effort. Although it is not too late to consider implementing a different approach, any such approach must have as high a probability of success as the current one and offer significant advantages (such as decreased cost). I argue here that the whole-genome shotgun proposed by Weber and Myers satisfies neither condition.