Infection with human rhinovirus 16 promotes enhanced IgE responsiveness in basophils of atopic asthmatics
Infection with human rhinovirus 16 promotes enhanced IgE responsiveness in basophils of atopic asthmatics
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DOI：
10.1111/cea.12390
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发表时间：
2014-10-01
影响因子：
6.1
通讯作者：
Woodfolk, J. A.
中科院分区：
文献类型：
--
作者：
Agrawal, R.;Wisniewski, J.;Woodfolk, J. A.
背景鼻病毒和IgE共同作用促进哮喘急性发作。虽然嗜碱性粒细胞是血液中被IgE激活的主要细胞类型，但它们在病毒诱导的哮喘发作中的作用仍然是难以捉摸的。在24小时PBMC培养物中的表面TSLP受体上。激活配置文件的嗜碱性粒细胞从特应性哮喘的挑战鼻内与人鼻病毒16进行监测，直接离体或在24小时的文化，在基线（0天），然后在第4天和第21天后challenge.ResultsBasophils在特应性哮喘，但不是在非特应性对照，上调TSLP受体后IgE受体连接。这种反应的程度与血清总IgE中变应原特异性的比例相关（r=0.615，P
BackgroundRhinovirus and IgE act in concert to promote asthma exacerbations. While basophils are the principal cell type in the blood that is activated by IgE, their role in virus-induced asthma episodes remains elusive.ObjectiveTo monitor IgE responsiveness in circulating basophils of rhinovirus-infected atopic asthmatics during acute infection and convalescence.MethodsThe capacity for basophils to respond to IgE was assessed by testing the effects of allergen, or cross-linking anti-Fc epsilon RI and anti-IgE antibodies, on surface TSLP receptor in 24-hour PBMC cultures. Activation profiles of basophils from atopic asthmatics challenged intranasally with human rhinovirus 16 were monitored directly ex vivo or else in 24-hour cultures, at baseline (day 0), and then at days 4 and 21 post-challenge.ResultsBasophils in atopic asthmatics, but not in non-atopic controls, upregulated TSLP receptor upon IgE receptor ligation. The magnitude of this response was correlated with the proportion of serum total IgE that was allergen-specific (r=0.615, P