Chromatin States in Mouse Sperm Correlate with Embryonic and Adult Regulatory Landscapes.
Chromatin States in Mouse Sperm Correlate with Embryonic and Adult Regulatory Landscapes.
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DOI：
10.1016/j.celrep.2017.01.034
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发表时间：
2017-02-07
期刊：
影响因子：
8.8
通讯作者：
Corces VG
中科院分区：
文献类型：
--
作者：
Jung YH;Sauria MEG;Lyu X;Cheema MS;Ausio J;Taylor J;Corces VG
哺乳动物精子基因组被认为缺乏大量的信息来调节受精后的未来表达。在这里，我们表明，大多数启动子在小鼠精子两侧的定位良好的核小体标记的活性组蛋白修饰。对这些修饰的分析表明，许多在胚胎和成人组织中起作用的增强子和超级增强子已经在精子中指定。精子基因组被CTCF和粘着蛋白结合在圆形精子细胞和胚胎干细胞（ESC）中也存在的位点上。这些位点介导将精子基因组组织成与mESC中发现的那些广泛重叠的结构域和区室的相互作用。这些结果表明，精子携带丰富的调控信息来源，部分编码的三维折叠指定的CTCF和cohesin。这一信息可能有助于未来的表达在胚胎和成年生活中，这表明环境对父系生殖系的影响是跨代传递的机制。
The mammalian sperm genome is thought to lack substantial information to regulate future expression after fertilization. Here we show that most promoters in mouse sperm are flanked by well-positioned nucleosomes marked by active histone modifications. Analysis of these modifications suggests that many enhancers and super-enhancers functional in embryonic and adult tissues are already specified in sperm. The sperm genome is bound by CTCF and cohesin at sites that are also present in round spermatids and embryonic stem cells (ESCs). These sites mediate interactions that organize the sperm genome into domains and compartments that overlap extensively with those found in mESCs. These results suggest that sperm carry a rich source of regulatory information, encoded in part by its three-dimensional folding specified by CTCF and cohesin. This information may contribute to future expression during embryonic and adult life, suggesting mechanisms by which environmental effects on the paternal germline are transmitted trans-generationally.