The type 1 transmembrane glycoprotein B7-H3 interacts with the glycolytic enzyme ENO1 to promote malignancy and glycolysis in HeLa cells.
The type 1 transmembrane glycoprotein B7-H3 interacts with the glycolytic enzyme ENO1 to promote malignancy and glycolysis in HeLa cells.
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1 型跨膜糖蛋白 B7-H3 与糖酵解酶 ENO1 相互作用，促进 HeLa 细胞的恶性肿瘤和糖酵解。
DOI：
10.1002/1873-3468.13164
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发表时间：
2018
期刊：
影响因子：
3.5
通讯作者：
Zhang Huizhong
中科院分区：
文献类型：
--
作者：
Zuo Jiahui;Wang Bowen;Long Min;Gao Zhaowei;Zhang Zhe;Wang Huiping;Wang Xi;Li Ruicheng;Dong Ke;Zhang Huizhong
1型跨膜糖蛋白B7-H3在肿瘤发生中的作用是有争议的;因此，更好地阐明其在癌症中的作用至关重要。在本研究中，发现79.3%的宫颈癌样本为B7-H3阳性，B7-H3的表达与样本的临床特征呈正相关。使用小干扰RNA沉默B7-H3或用细胞内ScFv阻断它减弱了HeLa细胞的恶性程度。通过在HeLa细胞中的下拉分析和液相色谱-质谱法，发现糖酵解酶ENO 1与B7-H3相互作用。随后，研究了B7-H3参与糖酵解的情况。我们观察到HeLa细胞中B7-H3下调后，ATP和乳酸盐以及c-Myc和乳酸脱氢酶A的水平降低。本研究的结果为B7‐H3介导肿瘤糖酵解提供了证据。
The role of the type 1 transmembrane glycoprotein B7‐H3 is controversial in tumorigenesis; thus, a better clarification of its involvement in cancer is crucial. In the present study, 79.3% of cervical cancer samples were found to be B7‐H3 positive and the expression of B7‐H3 was positively correlated with the clinical features of the samples. Silencing B7‐H3 using small interfering RNA or blocking it with intracellular ScFv attenuated the malignancy of HeLa cells. By pull‐down assay and liquid chromatography‐mass spectrometry in HeLa cells, the glycolytic enzyme ENO1 was found to interact with B7‐H3. Subsequently, the involvement of B7‐H3 in glycolysis was investigated. We observed decreases in the levels of ATP and lactate, as well as c‐Myc and lactate dehydrogenase A, upon B7‐H3 downregulation in HeLa cells. The results of the present study provide evidence for B7‐H3 mediating tumor glycolysis.