Cyanobacterial Enzymes for Bioalkane Production
Cyanobacterial Enzymes for Bioalkane Production
复制标题
DOI：
10.1007/978-981-13-0854-3_6
复制
发表时间：
2018-01-01
期刊：
影响因子：
--
通讯作者：
Kudo, Hisashi
中科院分区：
文献类型：
--
作者：
Arai, Munehito;Hayashi, Yuuki;Kudo, Hisashi
蓝藻生物合成烷烃是生产石油基燃料替代品的一种有吸引力的方式。蓝藻产生生物烷烃的关键酶是酰基-ACP还原酶（AAR）和脱氢脱甲酰加氧酶（ADO）。AAR催化脂肪酰基-ACP/CoA底物还原为脂肪醛，然后通过ADO转化为烷烃/烯烃。这些酶已被广泛用于通过蓝藻和其他生物的代谢工程生产生物燃料。然而，这两种蛋白质，特别是ADO，具有低的酶活性，并且它们的催化活性需要改进以用于生物燃料生产。近年来，AAR和ADO在基础科学和烷烃生产中的应用取得了进展。本章提供了一个概述的结构和功能的AAR和ADO，这些酶的蛋白质工程的研究的最新进展，以提高活性和修改底物的特异性，和蓝藻和其他生物体的代谢工程的例子，使用AAR和ADO的生物燃料生产。
Cyanobacterial biosynthesis of alkanes is an attractive way of producing substitutes for petroleum-based fuels. Key enzymes for bioalkane production in cyanobacteria are acyl-ACP reductase (AAR) and aldehyde-deformylating oxygenase (ADO). AAR catalyzes the reduction of the fatty acyl-ACP/CoA substrates to fatty aldehydes, which are then converted into alkanes/alkenes by ADO. These enzymes have been widely used for biofuel production by metabolic engineering of cyanobacteria and other organisms. However, both proteins, particularly ADO, have low enzymatic activities, and their catalytic activities are desired to be improved for use in biofuel production. Recently, progress has been made in the basic sciences and in the application of AAR and ADO in alkane production. This chapter provides an overview of recent advances in the study of the structure and function of AAR and ADO, protein engineering of these enzymes for improving activity and modifying substrate specificities, and examples of metabolic engineering of cyanobacteria and other organisms using AAR and ADO for biofuel production.