Invasion of vaginal epithelial cells by uropathogenic Escherichia coli
Invasion of vaginal epithelial cells by uropathogenic Escherichia coli
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DOI：
10.1038/s41467-020-16627-5
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发表时间：
2020-06-04
影响因子：
16.6
通讯作者：
Hadjifrangiskou, Maria
中科院分区：
文献类型：
--
作者：
Brannon, John R.;Dunigan, Taryn L.;Hadjifrangiskou, Maria
宿主相关的蓄水池是大多数反复和经常顽固性感染的原因。以前的研究证实，尿路感染的主要原因--致尿性大肠埃希菌可以在尿路感染之前附着在阴道上皮细胞上。在这里，我们证明了不同的尿液大肠杆菌分离株不仅附着，而且还入侵阴道细胞。在急性和慢性小鼠尿路感染模型中检测到阴道上皮细胞内定植，表明大肠杆菌在尿路感染后能够在阴道内居住。相反，在阴道定植模型中，在阴道细胞和尿路中检测到大肠杆菌，这表明阴道定植可以为膀胱播种。更关键的是，从有反复尿路感染病史的女性的临床样本中发现了阴道细胞内的细菌。这些发现表明，大肠杆菌可以建立一个阴道细胞内储存库，在引起向上感染之前，它可能安全地居住在那里，免受细胞外应激源的影响。
Host-associated reservoirs account for the majority of recurrent and oftentimes recalcitrant infections. Previous studies established that uropathogenic E. coli - the primary cause of urinary tract infections (UTIs) - can adhere to vaginal epithelial cells preceding UTI. Here, we demonstrate that diverse urinary E. coli isolates not only adhere to, but also invade vaginal cells. Intracellular colonization of the vaginal epithelium is detected in acute and chronic murine UTI models indicating the ability of E. coli to reside in the vagina following UTI. Conversely, in a vaginal colonization model, E. coli are detected inside vaginal cells and the urinary tract, indicating that vaginal colonization can seed the bladder. More critically, bacteria are identified inside vaginal cells from clinical samples from women with a history of recurrent UTI. These findings suggest that E. coli can establish a vaginal intracellular reservoir, where it may reside safely from extracellular stressors prior to causing an ascending infection.