A regulator that inhibits transcription by targeting an intersubunit interaction of the RNA polymerase holoenzyme
A regulator that inhibits transcription by targeting an intersubunit interaction of the RNA polymerase holoenzyme
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DOI：
10.1073/pnas.0400923101
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发表时间：
2004-03-30
影响因子：
11.1
通讯作者：
Hochschild, A
中科院分区：
文献类型：
--
作者：
Gregory, BD;Nickels, BE;Hochschild, A
细菌RNA聚合酶全酶的结构已经提供了关于全酶内亚单位间相互作用的详细信息。功能分析表明，其中之一在使全酶识别主要类型的细菌启动子方面是关键的。已有研究表明，这种涉及β亚基的翻盖结构域和Sigma亚基的保守区4的相互作用是一个潜在的调控靶点。在这里，我们提供了遗传和生化证据，表明Sigma区域4/β-Flat相互作用是转录因子Asia的靶标。具体地说，我们证明了亚洲直接与贝塔翻盖结合或区域4，从而通过破坏sigma区域4/贝塔翻盖相互作用来抑制转录启动。
The structures of the bacterial RNA polymerase holoenzyme have provided detailed information about the intersubunit interactions,within the holoenzyme. Functional analysis indicates that one of these is critical in enabling the holoenzyme to recognize the major class of bacterial promoters' It has been suggested that this interaction, involving the flap domain of the beta subunit and conserved region 4 of the sigma subunit, is a potential target for regulation. Here we provide genetic and biochemical evidence that the sigma region 4/beta-flap interaction is targeted by the transcription factor AsiA. Specifically, we show that AsiA competes directly with the beta-flap for binding to or region 4, thereby inhibiting transcription initiation by disrupting the sigma region 4/beta-flap interaction.