Drosophila RNase Z processes mitochondrial and nuclear pre-tRNA 3′ ends in vivo
Drosophila RNase Z processes mitochondrial and nuclear pre-tRNA 3′ ends in vivo
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DOI：
10.1093/nar/gkh182
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发表时间：
2004-01-01
影响因子：
14.9
通讯作者：
Marchfelder, A
中科院分区：
文献类型：
--
作者：
Dubrovsky, EB;Dubrovskaya, VA;Marchfelder, A
虽然正确的tRNA 3'末端对于蛋白质的生物合成至关重要，但真核生物中成熟tRNA 3'末端的产生知之甚少，迄今为止仅在体外进行了研究。本文首次报道了真核生物体内核糖核酸酶Z催化tRNA 3'末端成熟的作用。在果蝇S2培养细胞中，用RNAi在体内沉默JhI-1基因（RNase Z同源物）导致核和线粒体前tRNA的积累，表明JhI-1编码两种形式的tRNA 3'内切核酸酶RNase Z，并确定其在内切核酸酶tRNA 3'末端加工中的生物学作用。此外，我们的数据表明，在体内5'加工的核和线粒体前tRNA发生在3'加工。
Although correct tRNA 3' ends are crucial for protein biosynthesis, generation of mature tRNA 3' ends in eukaryotes is poorly understood and has so far only been investigated in vitro. We report here for the first time that eukaryotic tRNA 3' end maturation is catalysed by the endonuclease RNase Z in vivo. Silencing of the JhI-1 gene (RNase Z homolog) in vivo with RNAi in Drosophila S2 cultured cells causes accumulation of nuclear and mitochondrial pre-tRNAs, suggesting that JhI-1 encodes both forms of the tRNA 3' endonuclease RNase Z, and establishing its biological role in endonucleolytic tRNA 3' end processing. In addition our data show that in vivo 5' processing of nuclear and mitochondrial pre-tRNAs occurs before 3' processing.