Drug effects analysis on cells using a high throughput microfluidic chip
Drug effects analysis on cells using a high throughput microfluidic chip
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DOI：
10.1007/s10544-010-9486-2
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发表时间：
2011-02-01
影响因子：
2.8
通讯作者：
Que, Long
中科院分区：
文献类型：
--
作者：
Gong, Zhongcheng;Zhao, Hong;Que, Long
通常使用滴定板进行基于细胞的测定。由于化学化合物的大型库，需要稳健且快速的方法来以有效的方式发现、改进和测试潜在的候选药物。本文报道了利用微滴微流控芯片对人乳腺癌细胞进行药物效应分析。每个液滴用作纳升体积滴定板，并含有人乳腺癌细胞MDA-MB-231、细胞松弛素D药物溶液和细胞活力指示剂，如钙黄绿素AM，其发射细胞质绿色荧光。使用荧光显微镜并通过分析每个细胞的荧光图像来真实的实时监测药物对每个细胞的作用。经药物处理后，细胞形态和大小发生明显变化，与传统的培养皿技术相似，表明该方法为药物效应分析和高通量药物筛选和发现提供了一个潜在可行的技术平台。
Usually cell-based assay is performed using titer plates. Because of the large library of chemical compounds, robust and rapid methods are required to find, refine and test a potential drug candidate in an efficient manner. In this article, the drug effects analysis on human breast cancer cells with a droplet microfluidic chip is reported. Each droplet serves as a nanoliter-volume titer plate and contains a human breast cancer cell MDA-MB-231, Cytochalasin D drug solution and cell viability indicator such as Calcein AM, which emits cytoplasmic green fluorescence. The drug effects on each cell are monitored in real time using a fluorescence microscope and by analyzing the fluorescence image of each cell. Clear change of the cell shape and size has been observed after the drug treatment, which is similar to that of conventional petri dish technique, suggesting this approach is a potential viable technical platform for drug effect analysis and for high throughput drug screen and discovery.