IMMUNOELECTRON MICROSCOPY OF THE OUTER-MEMBRANE OF RAT HEPATOCYTE NUCLEAR ENVELOPES IN RELATION TO THE ROUGH ENDOPLASMIC-RETICULUM
IMMUNOELECTRON MICROSCOPY OF THE OUTER-MEMBRANE OF RAT HEPATOCYTE NUCLEAR ENVELOPES IN RELATION TO THE ROUGH ENDOPLASMIC-RETICULUM
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DOI：
10.1247/csf.8.1
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发表时间：
1983-01-01
影响因子：
1.5
通讯作者：
TASHIRO, Y
中科院分区：
文献类型：
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作者：
MATSUURA, S;MASUDA, R;TASHIRO, Y
大鼠肝脏经稀释戊二醛灌流后，分离出前置细胞核。这些核有时与粗面内质网(ER)联系，偶见外核膜与粗面ER膜连续。当这些前缀核与细胞色素P-450铁蛋白抗体结合物孵育时，外核膜的细胞质表面被铁蛋白标记，类似于粗大的内质网细胞膜的标记。在核孔区，铁蛋白颗粒存在于外膜上，直至孔洞复合体的外环。未固定核在0-4度孵育时。C或20度。C、核外核膜从核表面脱落。在这种情况下，核孔复合体仍然与内核膜相联系，并在这些孔区形成由内向外的小泡。在核基质中发现了一些被铁蛋白颗粒大量标记的粗糙小泡。它们可能来源于粗大的内质网，或来源于孵化过程中被人工内化的外核膜。
Prefixed nuclei were isolated from rat liver after perfusion with dilute glutaraldehyde. These nuclei sometimes were associated with the rough endoplasmic reticulum (ER), and occasionally continuity of the outer nuclear membrane with rough ER membrane was found. When these prefixed nuclei were incubated with ferritin antibody conjugates against cytochrome P-450, the cytoplasmic face of the outer nuclear membrane was labeled with ferritin similar to the labeling of the rough ER membrane. In the nuclear pore region, ferritin particles were present on the outer membrane up to the outer annuli of the pore complexes. When unfixed nuclei were incubated at 0-4.degree. C or at 20.degree. C, some outer nuclear membranes were detached from the nuclear surface. In this case the nuclear pore complexes remained associated with the inner nuclear membrane, and small inside-out vesicles were formed at these pore regions. Some rough vesicles heavily labeled with ferritin particles were found within the nuclear matrix. They probably were derived from the rough ER or from the outer nuclear envelope which was internalized artificially during incubation.