Stomatal Bioassay to Characterize Bacterial-Stimulated PTI at the Pre-Invasion Phase of Infection.
Stomatal Bioassay to Characterize Bacterial-Stimulated PTI at the Pre-Invasion Phase of Infection.
复制标题
气孔生物测定来表征感染前入侵阶段细菌刺激的 PTI。
DOI：
10.1007/978-1-4939-6859-6_19
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发表时间：
2017
期刊：
影响因子：
--
通讯作者：
Melotto,Maeli
中科院分区：
文献类型：
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作者：
Montano,Jeanine;Melotto,Maeli
细菌触发的气孔关闭是植物免疫的功能输出，也称为气孔防御。这是由植物病原体识别受体 (PRR) 识别病原体相关分子模式 (PAMP) 介导的早期反应。已经描述了几种分析细菌响应气孔运动的方法，但微调实验程序的困难仍然存在。在这里，我们提供了一种通过高质量显微成像评估气孔防御的详细方法，并解释了获取可靠数据的故障排除步骤。尽管此过程需要对叶子样本进行最少的操作，但控制所有可能干扰气孔运动的环境条件和外在变量至关重要。这里描述的方法也适用于新病理系统中气孔反应的体内表征，并且可以与其他分析测定结合使用，以获得对早期 PAMP 触发免疫 (PTI) 的详细了解。
Bacterium-triggered stomatal closure is a functional output of plant immunity, also known as stomatal defense. This is an early response mediated by the recognition of pathogen-associated molecular patterns (PAMPs) by the plant’s pathogen recognition receptors (PRRs). Several approaches to analyzing stomatal movement in response to bacteria have been described, but difficulties in fine-tuning the experimental procedures still exist. Here we provide a detailed method for assessing stomatal defense via high-quality microscopic imaging and explain trouble-shooting steps to obtaining robust data. Although this procedure requires minimal manipulation of the leaf sample, it is crucial to control all environmental conditions and extrinsic variables that could interfere with stomatal movement. The method described here is also suitable for in vivo characterization of stomatal response in new pathosystems and can be used in conjunction with other profiling assays to gain a detailed understanding of early PAMP-triggered immunity (PTI).