Rescue of atretic follicles in vitro and in vivo.
Rescue of atretic follicles in vitro and in vivo.
复制标题
体外和体内闭锁卵泡的拯救。
DOI：
10.1095/biolreprod40.1.181
复制
发表时间：
1989
影响因子：
3.6
通讯作者：
Hirshfield,AN
中科院分区：
文献类型：
--
作者：
Hirshfield,AN
本研究的目的是确定闭锁卵泡是否可以被挽救并恢复排卵发育途径。大鼠连续输注3h -胸腺嘧啶（3H-TdR），使健康的窦腔卵泡均匀标记，而闭锁的窦腔卵泡不均匀标记。然后停止输注，大鼠接受已知的刺激卵泡再生的实验性治疗。用妊娠母马血清促性腺激素（PMSG）刺激未成熟大鼠超排卵。成年大鼠被阉割，以刺激剩余卵巢的代偿性卵泡发育。此外，体外培养成年大鼠单个卵泡颗粒细胞。在治疗期结束时观察到的差异标记模式，用于确定在治疗期开始时卵泡的状况。稀疏标记的细胞培养物被发现，表明一些来自闭锁卵泡的细胞能够在组织培养中建立。然而，没有证据表明闭锁卵泡在体内恢复。所有通过PMSG或半胱氨酸募集的卵泡都被大量和均匀地标记。所有标记不良的卵泡显然仍在继续变性过程。这些观察结果表明，尽管一些颗粒细胞在闭锁卵泡中继续存活，但一旦卵泡在体内开始退化，它可能不会回到排卵途径。
The purpose of this work was to determine if atretic follicles could be rescued and could return to the ovulatory pathway of development. Rats were given continuous infusions of3H-thymidine (3H-TdR) resulting in uniform labeling of healthy antral follicles versus patchy labeling of atretic antral follicles. The infusion was then stopped and rats were subjected to experimental treatments known to stimulate follicular recruitment. Immature rats were given injections of pregnant mare's serum gonadotropin (PMSG) to provoke super-ovulation. Adult rats were hemicastrated to provoke compensatory follicular development in the remaining ovary. In addition, granulosa cells from individual follicles of adult rats were cultured in vitro. The differential labeling patterns, observed at the end of the treatment period, were used to determine, a posteriori, the condition of follicles as they had been at the start of the treatment period. Sparsely labeled cell cultures were found, indicating that some cells from atretic follicles were able to become established in tissue culture. However, there was no evidence that atretic follicles bad revived in vivo. All follicles recruited for ovulation by PMSG or hemicastration were heavily and uniformly labeled. All poorly labeled follicles were clearly continuing their process of degeneration. These observations suggest that, despite continued viability of some granulosa cells in atretic follicles, once a follicle begins to degenerate in vivo, it will probably not return to the ovulatory pathway.