Cloning and expression analysis of CaPIP1-1 gene in pepper (Capsicum annuum L.).
Cloning and expression analysis of CaPIP1-1 gene in pepper (Capsicum annuum L.).
复制标题
DOI：
10.1016/j.gene.2015.03.012
复制
发表时间：
2015-05
期刊：
影响因子：
3.5
通讯作者：
Yanxu Yin;Shu-Bin Wang;Huai-Xia Zhang;Huaijuan Xiao;Jing-Hao Jin;J-J Ji-J;Hua Jing;Rugang Chen
中科院分区：
文献类型：
--
作者：
Yanxu Yin;Shu-Bin Wang;Huai-Xia Zhang;Huaijuan Xiao;Jing-Hao Jin;J-J Ji-J;Hua Jing;Rugang Chen
植物水通道蛋白负责水跨膜运输，在非生物和生物胁迫下发挥重要作用。根据辣椒疫病菌接种转录组数据库和冷胁迫文库，从辣椒P70中分离得到一种新型质膜内蛋白CaPIP1-1。CaPIP1-1全长1155bp，开放阅读框861bp，编码286个氨基酸。在CaPIP1-1 中观察到三个内含子，表现出CT/AC 剪接连接。 CaPIP1-1内含子的数量和位置与番茄和马铃薯中观察到的相同。CaPIP1-1在辣椒果实中大量表达。 CaPIP1-1在不同胁迫下转录水平升高，包括冷胁迫、盐胁迫、甘露醇胁迫、水杨酸、ABA处理和辣椒疫霉感染。 50 μM HgCl2 和 100 μM 氟啶酮可下调 CaPIP1-1 的表达。辣椒植物沉默了 cv. 中的 CaPIP1-1。采用离体叶法，切门表现出生长抑制和对盐和甘露醇胁迫的耐受性降低。
Plant aquaporins are responsible for water transmembrane transport, which play an important role on abiotic and biotic stresses. A novel plasma membrane intrinsic protein ofCaPIP1-1was isolated from the pepper P70 according to transcriptome databases of Phytophthora capsici inoculation and chilling stress library.CaPIP1-1, which is 1155 bp in length with an open reading frame of 861 bp, encoded 286 amino acids. Three introns, exhibited CT/AC splice junctions, were observed inCaPIP1-1. The numbers and location of introns inCaPIP1-1were the same as observed in tomato and potato.CaPIP1-1was abundantly expressed in pepper fruit. Increased transcription levels ofCaPIP1-1were found in the different stresses, including chilling stress, salt stress, mannitol stress, salicylic acid, ABA treatment andPhytophthora capsiciinfection. The expression ofCaPIP1-1was downregulated by 50 μM HgCl2and 100 μM fluridone. The pepper plants silencedCaPIP1-1in cv. Qiemen showed growth inhibition and decreased tolerance to salt and mannitol stresses using detached leaf method.