Selective transport of cationized fluorescent topoisomerase into nuclei of live cells for DNA damage studies.
Selective transport of cationized fluorescent topoisomerase into nuclei of live cells for DNA damage studies.
复制标题
将阳离子荧光拓扑异构酶选择性转运至活细胞细胞核中，用于 DNA 损伤研究。
DOI：
10.1007/978-1-62703-706-8_14
复制
发表时间：
2014
期刊：
影响因子：
--
通讯作者：
Didenko,VladimirV
中科院分区：
文献类型：
--
作者：
Minchew,CandaceL;Didenko,VladimirV
将荧光标记的DNA修饰蛋白靶向递送到细胞核中允许研究活细胞中的DNA损伤和染色质功能。可商购获得的蛋白质递送载体不能提供选择性核内运输，并且主要在细胞质中卸载它们的货物。在这里，我们描述了一个简单的方法，特异性的核内转运牛痘拓扑异构酶蛋白的基础上，其阳离子化。可以通过荧光显微镜观察和监测递送的蛋白质。该技术具有成本效益和节省时间。它可以用于活细胞研究。
The targeted delivery of fluorescently labeled, DNA-modifying proteins into cellular nuclei permits investigation of DNA damage and chromatin function in living cells. Commercially available protein delivery vectors cannot provide selective intranuclear transportation and primarily unload their cargo in the cytoplasm. Here we describe a simple approach for specific intranuclear transportation of vaccinia topoisomerase protein based on its cationization. The delivered protein can be observed and monitored by fluorescence microscopy. The technique is cost-efficient and time-saving. It can be useful in live cell studies.