Age-associated alterations in hepatic glutathione-S-transferase activities.
Age-associated alterations in hepatic glutathione-S-transferase activities.
复制标题
肝谷胱甘肽-S-转移酶活性与年龄相关的变化。
DOI：
10.1016/0006-2952(85)90440-x
复制
发表时间：
1985
影响因子：
5.8
通讯作者：
Kenichi Kitani
中科院分区：
文献类型：
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作者：
Shoichi Fujita;H. Kitagawa;Harumi Ishizawa;Tokuji Suzuki;Kenichi Kitani
在1.5 ~ 28月龄的雌雄Fischer-344大鼠中，研究了肝细胞溶质谷胱甘肽-S-转移酶（GST）对四水合磺溴酞钠（BSP）、氧化苯乙烯（STOX）、反式-4-苯基-3-丁烯-2-酮（PBO）、1，2-二氯-4-硝基苯（DCNB）和1-氯-2，4-二硝基苯（CDNB）的活性变化。雄性大鼠PBO和DCNB的GST活性随月龄增加而增加，6-12月龄时达到最大值，随后逐渐下降，28月龄时降至最低。STOX和BSP的GST活性在1.5个月后没有表现出任何显著的增加，直到12个月都保持在这个水平，然后逐渐下降，直到28个月时达到最低值，而雄性大鼠的CDNB的GST活性没有表现出明显的年龄相关性变化。女性GST活性的变化明显小于男性。GST活性的性别差异（雄性显著高于雌性），GST活性的动力学研究表明，GST同工酶的相对丰度和总量的变化导致GST活性随年龄的底物选择性变化。
Age-associated alterations of hepatic cytosolic glutathione-S-transferase (GST) activities towards sulfobromophthalein sodium tetrahydrate (BSP), styrene oxide (STOX), trans-4-phenyl-3-butene-2-one (PBO),1,2-dichloro-4-nitrobenzene (DCNB), and 1-chloro-2,4-dinitrobenzene (CDNB) were investigated in Fischer-344 rats of both sexes with ages ranging from 1.5 to 28 months. The GST activities towards PBO and DCNB in male rats increased with age till 6–12 months when maximum values were attained, and then gradually decreased till 28 months when the values became the lowest. The GST activities towards STOX and BSP did not show any significant increase after 1.5 months and stayed at this level till 12 months, followed by a gradual decrease till 28 months when the values were the lowest.In contrast, the GST activity towards CDNB in male rats did not show much of an age-associated alteration. Age-associated alterations in GST activities in females Were much smaller than those observed in males. Sex differences in GST activities (significantly higher male values than female values) were observed with all the substrates examined at least at some time of the animal life.The kinetic studies of GST activities indicated that alterations in the relative abundance as well as the total quantity of GST isozymes caused the substrate selective alterations of GST activities with age.