Effectively Improve the Astaxanthin Production by Combined Additives Regulating Different Metabolic Nodes in Phaffia rhodozyma.
Effectively Improve the Astaxanthin Production by Combined Additives Regulating Different Metabolic Nodes in Phaffia rhodozyma.
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DOI：
10.3389/fbioe.2021.812309
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发表时间：
2021
影响因子：
5.7
通讯作者：
Li L
中科院分区：
文献类型：
--
作者：
Li Z;Yang H;Zheng C;Du X;Ni H;He N;Yang L;You L;Zhu Y;Li L
虾青素是一种重要的天然资源，广泛存在于海洋环境中。代谢调控是提高红酵母虾青素产量的有效方法。大多数研究都集中在单一的监管机构，其影响有限。在这项研究中，16代谢调节剂进行了筛选，以提高虾青素产量的高产菌株和野生型。氟康唑和谷氨酸分别使MVP 14中的虾青素体积产率增加25.8%和30.9%，而乙醇使DSM 626中的虾青素体积产率增加29.3%。此外，还筛选出6种抑制竞争途径、促进主要途径合成的添加剂进行组合处理。我们发现，最佳组合是青霉素，乙醇，三氯生，氟康唑，提高了51%的虾青素细胞产量。因此，我们建议，同时促进主途径（甲羟戊酸）和抑制竞争途径（脂肪酸合成和麦角固醇）是提高虾青素细胞产量的最佳策略。此外，应避免生物质途径的调节剂以提高细胞产率。本研究为红酵母中虾青素的开发利用提供了技术依据。
Astaxanthin is an important natural resource that is widely found in marine environments. Metabolic regulation is an effective method for improving astaxanthin production in Phaffia rhodozyma. Most studies have focused on single regulators, which have limited effects. In this study, 16 metabolic regulators were screened to improve astaxanthin production in high-yield and wild-type strains. Fluconazol and glutamic acid increased astaxanthin volumetric yield in MVP14 by 25.8 and 30.9%, respectively, while ethanol increased astaxanthin volumetric yield in DSM626, 29.3%. Furthermore, six additives that inhibit the competing pathways and promote the main pathway for astaxanthin synthesis were selected for combination treatment. We found that the optimal combination was penicillin, ethanol, triclosan, and fluconazol, which increased astaxanthin cell yield by 51%. Therefore, we suggest that simultaneously promoting the master pathways (mevalonate) and inhibiting competing pathways (fatty acid synthesis and ergosterol) is the best strategy to improve astaxanthin cell yield. Moreover, regulators of the biomass pathway should be avoided to improve cell yield. This study provides a technical basis for the utilisation of astaxanthin in P. rhodozyma.