Biomarkers of Dose and Effect of Inhaled Ozone in Resting versus Exercising Human Subjects: Comparison with Resting Rats.
Biomarkers of Dose and Effect of Inhaled Ozone in Resting versus Exercising Human Subjects: Comparison with Resting Rats.
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DOI：
10.4137/bmi.s11102
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发表时间：
2013
影响因子：
3.8
通讯作者：
Devlin R
中科院分区：
文献类型：
--
作者：
Hatch GE;McKee J;Brown J;McDonnell W;Seal E;Soukup J;Slade R;Crissman K;Devlin R
为了确定运动对吸入污染物的肺剂量的影响，我们比较了人类运动水平之间以及人类和大鼠之间的吸入臭氧（O3）剂量和毒性效应的生物标志物。将静息的人类受试者暴露于标记O3（18 O3，0.4 ppm，持续2小时），并测量肺泡O3剂量，作为鼻、支气管和支气管肺泡灌洗液（BALF）细胞和细胞外物质中过量18 O的浓度。我们将O3剂量与BALF中可测量的效应（BALF蛋白、LDH、IL-6和抗氧化物质的变化）相关。一项对静息受试者的平行研究检查了O3后肺功能（FEV 1）的变化。受试者在休息时暴露的BALF细胞中的18 O浓度是运动受试者的1/5，与暴露期间呼吸的O3量成正比。先前在运动受试者中观察到的肺泡O3剂量和毒性的定量测量在O3暴露的静息受试者中大大减少或不可观察。休息的大鼠和休息的人类被发现有类似的肺泡O3剂量。
To determine the influence of exercise on pulmonary dose of inhaled pollutants, we compared biomarkers of inhaled ozone (O3) dose and toxic effect between exercise levels in humans, and between humans and rats. Resting human subjects were exposed to labeled O3 (18O3, 0.4 ppm, for 2 hours) and alveolar O3 dose measured as the concentration of excess 18O in cells and extracellular material of nasal, bronchial, and bronchoalveolar lavage fluid (BALF). We related O3 dose to effects (changes in BALF protein, LDH, IL-6, and antioxidant substances) measurable in the BALF. A parallel study of resting subjects examined lung function (FEV1) changes following O3. Subjects exposed while resting had 18O concentrations in BALF cells that were 1/5th of those of exercising subjects and directly proportional to the amount of O3 breathed during exposure. Quantitative measures of alveolar O3 dose and toxicity that were observed previously in exercising subjects were greatly reduced or non-observable in O3 exposed resting subjects. Resting rats and resting humans were found to have a similar alveolar O3 dose.