Screening of immunomodulatory and adhesive Lactobacillus with antagonistic activities against Salmonella from fermented vegetables
Screening of immunomodulatory and adhesive Lactobacillus with antagonistic activities against Salmonella from fermented vegetables
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发酵蔬菜中具有拮抗沙门氏菌活性的免疫调节粘附性乳杆菌的筛选
DOI：
10.1007/s11274-015-1939-6
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发表时间：
2015-12-01
影响因子：
4.1
通讯作者：
Zhao, Xin
中科院分区：
文献类型：
--
作者：
Feng, Junchang;Liu, Pilong;Zhao, Xin
本研究的目的是通过乳酸菌的体外粘附和免疫调节特性来筛选益生菌。本研究首先从发酵蔬菜（酸菜、豆类和卷心菜）中随机选择16株LAB菌株，筛选其对酸、胆盐、胃蛋白酶和胰酶的耐受性、细菌抑制活性和对Caco-2细胞的粘附能力。然后，选择粘附能力最强的4株菌株，在体外进一步研究其免疫调节特性和抑制沙门氏菌对Caco-2细胞的粘附和侵袭作用。结果表明，这16株乳酸菌在模拟胃肠道条件下均能有效存活，并能抑制6种被试病原体的生长。鼠李糖乳杆菌P1、植物乳杆菌P2、鼠李糖乳杆菌P3和干酪乳杆菌P4对Caco-2细胞的粘附能力最强。此外，植物乳杆菌P2菌株具有较强的诱导脾单核细胞表达肿瘤坏死因子- α和白细胞介素-12的能力，并能强烈抑制肠炎沙门氏菌ATCC13076对Caco-2细胞的粘附和侵袭。上述结果提示P2乳杆菌可作为食品中抗沙门氏菌感染的候选益生菌。
The purpose of this study was to select strains of lactic acid bacteria (LAB) by their in vitro adhesive and immunomodulatory properties for potential use as probiotics. In this study, 16 randomly selected LAB strains from fermented vegetables (sauerkraut, bean and cabbage) were first screened for their tolerance to acid, bile salts, pepsin and pancreatin, bacterial inhibitory activities and abilities to adherence to Caco-2 cells. Then, 4 strains with the highest adhesion abilities were selected for further studies of their immunomodulatory properties and inhibitory effects against Salmonella adhesion and invasion to Caco-2 cells in vitro. The results showed that these 16 LAB strains effectively survived in simulated gastrointestinal condition and inhibited growth of six tested pathogens. Lactobacillus rhamnosus P1, Lactobacillus plantarum P2, Lactobacillus rhamnosus P3 and Lactobacillus casei P4 had the highest abilities to adhere to Caco-2 cells. Furthermore, L. plantarum P2 strain showed higher abilities to induce expression of tumor necrosis factor-alpha and interleukin-12 by splenic monocytes and strongly inhibited the adhesion and invasion of S. enteritidis ATCC13076 to Caco-2 cells. These results suggest that Lactobacillus strains P2 could be used as a probiotic candidate in food against Salmonella infection.