Serous Membrane Detachment with Ultrasonic Homogenizer Improves Engraftment of Fetal Liver to Liver Surface in a Rat Model of Cirrhosis.
Serous Membrane Detachment with Ultrasonic Homogenizer Improves Engraftment of Fetal Liver to Liver Surface in a Rat Model of Cirrhosis.
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DOI：
10.3390/ijms222111589
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发表时间：
2021-10-27
影响因子：
5.6
通讯作者：
Taniguchi H
中科院分区：
文献类型：
--
作者：
Kawakatsu-Hatada Y;Murata S;Mori A;Kimura K;Taniguchi H
肝移植是治疗终末期肝硬化最有效的方法。然而，由于严重的供体短缺，迫切需要新的治疗方法来取代肝移植。最近的研究集中在使用肝细胞和诱导多能干细胞的新治疗方法上，我们努力开发将这些细胞移植到肝脏表面的方法。在本研究中，我们评估了几种方法的效率，在剥离浆膜覆盖的肝表面移植到肝表面。通过各种方法分离肝硬化模型中二肽基肽酶IV（DPPIV）缺陷大鼠的肝表面，然后移植DPPIV阳性大鼠的胎肝。我们发现，与未脱离相比，在用超声均质器（其模拟Cavitron Ultrasonic Surgical Aspirator®（CUSA））进行浆膜脱离后接受移植的大鼠中，植入率和面积以及肝功能得到改善。超声均质器法出血量明显低于针刺法和电刀法。这些发现提供了证据，表明使用CUSA移植到具有浆膜脱离的肝表面可能有助于开发使用细胞或组织的肝硬化新治疗方法。
Liver transplantation is the most effective treatment for end-stage cirrhosis. However, due to serious donor shortages, new treatments to replace liver transplantation are sorely needed. Recent studies have focused on novel therapeutic methods using hepatocytes and induced pluripotent stem cells, we try hard to develop methods for transplanting these cells to the liver surface. In the present study, we evaluated several methods for their efficiency in the detachment of serous membrane covering the liver surface for transplantation to the liver surface. The liver surface of dipeptidyl peptidase IV (DPPIV)-deficient rats in a cirrhosis model was detached by various methods, and then fetal livers from DPPIV-positive rats were transplanted. We found that the engraftment rate and area as well as the liver function were improved in rats undergoing transplantation following serous membrane detachment with an ultrasonic homogenizer, which mimics the Cavitron Ultrasonic Surgical Aspirator® (CUSA), compared with no detachment. Furthermore, the bleeding amount was lower with the ultrasonic homogenizer method than with the needle and electric scalpel methods. These findings provide evidence that transplantation to the liver surface with serous membrane detachment using CUSA might contribute to the development of new treatments for cirrhosis using cells or tissues.
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