Effect of organ-specific fibroblasts on proliferation and differentiation of breast cancer cells
Effect of organ-specific fibroblasts on proliferation and differentiation of breast cancer cells
复制标题
器官特异性成纤维细胞对乳腺癌细胞增殖和分化的影响
DOI：
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发表时间：
2005
影响因子：
3.8
通讯作者：
K. Hirakawa
中科院分区：
文献类型：
--
作者：
M. Yashiro;K. Ikeda;M. Tendo;T. Ishikawa;K. Hirakawa
乳腺癌包含肿瘤细胞和基质细胞，包括成纤维细胞、内皮细胞和淋巴细胞。乳腺癌细胞的增殖可能部分受间充质细胞，尤其是成纤维细胞的控制。我们在体内研究了成纤维细胞对乳腺癌细胞致瘤性和组织学特征的影响，并在体外分析了成纤维细胞产生的生长促进因子。使用来自四种细胞系的乳腺癌细胞和来自四名乳腺癌患者皮肤和乳腺组织细胞系的成纤维细胞。取适量乳腺肿瘤细胞和成纤维细胞皮下接种裸鼠；对产生的肿瘤进行检查。然后将成纤维细胞的条件培养基加入到乳腺癌细胞培养中，研究其生长作用，并分析乳腺成纤维细胞的生长促进因子。与单独接种乳腺癌细胞相比，将乳腺癌细胞与乳腺成纤维细胞共同接种小鼠可显著增加肿瘤的致瘤性和肿瘤的大小。组织学上，与乳腺成纤维细胞共接种形成的肿瘤表现为广泛纤维化的剑鞘型，而单独注射乳腺癌细胞或与皮肤成纤维细胞共接种形成的肿瘤表现为实性。乳腺成纤维细胞培养液显著提高了乳腺癌细胞的体外生长，而各种皮肤成纤维细胞并没有显示出这种效果。器官特异性成纤维细胞与乳腺癌细胞之间的结构和功能相互作用可能对乳腺癌的生长和进展起着重要的调节作用。
SummaryBreast carcinomas contain both tumor cells and stromal cells, including fibroblasts, endothelial cells, and lymphocytes. Proliferation of breast cancer cells may be controlled partly by mesenchymal cells, especially fibroblasts. We studied effects of fibroblasts on tumorigenicity and histologic features of breast cancer cells vivo, and analyzed fibroblast-produced growth-promoting factors in vitro. Breast carcinoma cells from four lines, and fibroblasts from lines obtained from skin and breast tissue of four patients with breast cancer were used. A suitable number of breast tumor cells and fibroblasts were inoculated subcutaneously into nude mice; resulting tumors were examined. Then conditioned medium from fibroblasts was added to cultures of breast cancer cells to study growth effects, and growth-promoting factors from breast fibroblasts were analyzed. Co-inoculation of breast cancer cells with breast fibroblasts into mice significantly increased tumorigenicity and tumor size beyond those obtained with breast cancer cells alone. Histologically, tumors resulting from co-inoculation with breast fibroblasts showed a scirrhous pattern with extensive fibrosis, while those formed by breast cancer cells injected alone or co-inoculation with skin fibroblasts showed a solid pattern. Medium from breast fibroblasts significantly increased breast cancer cell growth in vitro, while the various skin fibroblasts did not all show this effect. Structural and functional interactions between organ-specific fibroblasts and breast cancer cells may importantly regulate breast cancer growth and progression.
影响因子：
11.2
作者：
Jian Zhou;B. Gurates;Sijun Yang;Siby Sebastian;S. Bulun
通讯作者：
Jian Zhou;B. Gurates;Sijun Yang;Siby Sebastian;S. Bulun
DOI：
10.1073/pnas.87.1.75
发表时间：
1990-01-01
影响因子：
11.1
作者：
CAMPS, JL;CHANG, SM;CHUNG, LWK
通讯作者：
CHUNG, LWK