A cellular memory module conveys epigenetic inheritance of hedgehog expression during Drosophila wing imaginal disc development
A cellular memory module conveys epigenetic inheritance of hedgehog expression during Drosophila wing imaginal disc development
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DOI：
10.1101/gad.242702
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发表时间：
2002-10-15
影响因子：
10.5
通讯作者：
Paro, R
中科院分区：
文献类型：
--
作者：
Maurange, C;Paro, R
在果蝇中，Trithorax-group （trxG）和Polycomb-group （PcG）蛋白与染色体元件相互作用，称为细胞记忆模块（CMMs）。通过修改染色质，这确保了发育调节因子转录状态的稳定可遗传维持，如胚胎时期定义的同质基因。我们想知道在幼虫发育过程中，这些cmm是否也能控制与图像盘图案相关的基因的表达。我们的研究结果表明，刺猬基因的表达，一旦激活，是由一个CMM维持。此外，我们的实验表明，与胚胎阶段相反，这些cmm在幼虫阶段切换到激活状态可能需要特定的反式激活剂。我们的研究结果表明，细胞在特定发育领域的模式不仅依赖于来自形态因子的外部线索，而且还取决于细胞以前的历史，因为cmm的控制确保了预格式化的基因表达模式。
In Drosophila, the Trithorax-group (trxG) and Polycomb-group (PcG) proteins interact with chromosomal elements, termed Cellular Memory Modules (CMMs). By modifying chromatin, this ensures a stable heritable maintenance of the transcriptional state of developmental regulators, like the homeotic genes, that is defined embryonically. We asked whether such CMMs could also control expression of genes involved in patterning imaginal discs during larval development. Our results demonstrate that expression of the hedgehog gene, once activated, is maintained by a CMM. In addition, our experiments indicate that the switching of such CMMs to an active state during larval stages, in contrast to embryonic stages, may require specific trans-activators. Our results suggest that the patterning of cells in particular developmental fields in the imaginal discs does not only rely on external cues from morphogens, but also depends on the previous history of the cells, as the control by CMMs ensures a preformatted gene expression pattern.