Expression of mRNA encoding basic fibroblast growth factor (bFGF) in bovine corpora lutea and cultured luteal cells.
Expression of mRNA encoding basic fibroblast growth factor (bFGF) in bovine corpora lutea and cultured luteal cells.
复制标题
DOI：
10.1530/jrf.0.0910001
复制
发表时间：
1991
期刊：
影响因子：
--
通讯作者：
D. Stirling;Michael R. Waterman;Evan R. Simpson
中科院分区：
文献类型：
--
作者：
D. Stirling;Michael R. Waterman;Evan R. Simpson
以牛碱性成纤维细胞生长因子（bovine basic fibroblast growth factor，bFGF）mRNA的1.4kb互补cDNA为模板，合成了放射性标记的cRNA，并以此为探针，研究了bFGF mRNA在牛卵巢组织和原代培养的黄体细胞中的表达。北方分析的多聚（A +）RNA制备的卵泡和黄体的各个阶段揭示了一个主要的mRNA种类的7 kb的黄体的所有阶段，其量较高的黄体期后期。在任何大小的卵泡中均未检测到可杂交信息。当在原代培养中建立黄体细胞时，维持7 kb mRNA种类的表达。LH/hCG或Bt_2cAMP处理细胞后，其表达明显增加。前列腺素F-2 α处理导致该7 kb mRNA的基础含量显著降低，并且还严重损害了LH刺激该表达的能力。
A radiolabelled cRNA was synthesized using a 1.4 kb cDNA complementary to mRNA encoding bovine basic fibroblast growth factor (bFGF) as a template, and used as a probe to investigate the expression of mRNA encoding bFGF in bovine ovarian tissue, and luteal cells in primary culture. Northern analysis of poly(A +)RNA prepared from follicles and corpora lutea of various stages revealed a major mRNA species of 7 kb in corpora lutea of all stages, the amount of which was higher late in the luteal phase. No hybridizable message was detectable in follicles of any size. When luteal cells were established in primary culture, expression of the 7 kb mRNA species was maintained. This expression was increased markedly when cells were treated with LH/hCG or Bt2cAMP. Prostaglandin F-2 alpha treatment caused a marked decrease in the basal content of this 7 kb mRNA, and also severely impaired the ability of LH to stimulate this expression.