Transitin, a nestin-related intermediate filament, is expressed by neural progenitors and can be induced in Muller glia in the chicken retina
Transitin, a nestin-related intermediate filament, is expressed by neural progenitors and can be induced in Muller glia in the chicken retina
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DOI：
10.1002/cne.20406
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发表时间：
2005-03-28
影响因子：
2.5
通讯作者：
Omar, G
中科院分区：
文献类型：
--
作者：
Fischer, AJ;Omar, G
本研究的目的是测试是否transitin，巢蛋白的鸟类同源物，在发育和出生后的鸡视网膜祖细胞的表达。由于nestin已被广泛用作哺乳动物神经系统中神经前体细胞的细胞区分标记物，我们期望找到视网膜神经前体细胞特异性表达的transitin。在发育的早期阶段，过渡蛋白由视网膜中的神经祖细胞和发育中的睫状体中的细胞表达。在发育的后期阶段，过渡蛋白表达持续分化穆勒神经胶质细胞，但随着成熟的进行，这些细胞下调。在出生后的小鸡，transitin的表达仅限于在视网膜的周边边缘的神经祖细胞。我们发现，在成熟的Muller胶质细胞的表达的transitin诱导眼内注射胰岛素和成纤维细胞生长因子-2（FGF 2），但不睫状神经营养因子。响应于胰岛素和FGF 2，在睫状体的非色素上皮（NPE）中诱导了transitin的表达。在出生后的视网膜中，急性视网膜损伤瞬时诱导Muller胶质细胞中的transitin表达。我们建议，在出生后的动物视网膜Muller神经胶质细胞和NPE细胞表达的transitin代表了去分化的状态，并朝着成为神经源性祖细胞的一步。总之，我们的研究结果表明，在胚胎和出生后的鸡视网膜神经祖细胞表达的transitin。然而，transitin并不完全由神经祖细胞表达，也由非神经源性细胞表达。(C)2005 Wiley-Liss，Inc.
The purpose of this study was to test whether transitin, the avian homologue of nestin, is expressed by retinal progenitors in the developing and postnatal chicken. Because nestin has been widely used as a cell-distinguishing marker of neural progenitors in the mammalian nervous system, we expected to find transitin expressed specifically by the neural progenitors of the retina. In early stages of development, transitin is expressed by neural progenitors in the retina and by cells in the developing ciliary body. During later stages of development, transitin expression persists in differentiating Muller glia but is down-regulated by these cells as maturation proceeds. In the postnatal chick, transitin expression is restricted to neural progenitors at the peripheral edge of the retina. We found that the expression of transitin in mature Muller glia was induced by intraocular injections of insulin and fibroblast growth factor-2 (FGF2) but not by ciliary neurotrophic factor. In response to insulin and FGF2, the expression of transitin was induced in the nonpigmented epithelium (NPE) of the ciliary body. In the postnatal retina, acute retinal damage transiently induces transitin expression in Muller glia. We propose that the expression of transitin by retinal Muller glia and NPE cells in the postnatal animal represents a state of de-differentiation and a step toward becoming neurogenic progenitor cells. Taken together, our findings indicate that transitin is expressed by neural progenitors in the embryonic and postnatal chicken retina. However, transitin is not exclusively expressed by neural progenitors and is also expressed by non-neurogenic cells. (C) 2005 Wiley-Liss, Inc.