Isolation of a novel population of multipotent adult stem cells from human hair follicles
Isolation of a novel population of multipotent adult stem cells from human hair follicles
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DOI：
10.2353/ajpath.2006.051170
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发表时间：
2006-06-01
影响因子：
6
通讯作者：
Xu, Xiaowei
中科院分区：
文献类型：
--
作者：
Yu, Hong;Fang, Dong;Xu, Xiaowei
众所周知，毛囊含有一个成熟干细胞的特征性小生境：凸起，其中含有上皮干细胞和黑素细胞干细胞。使用人胚胎干细胞培养条件，我们从人毛囊中分离出一群成体干细胞，这些干细胞与已知的上皮干细胞或黑素细胞干细胞明显不同。这些细胞不表达鳞状细胞或黑色素细胞标记物，但表达神经嵴和神经元干细胞标记物以及胚胎干细胞转录因子Nanog和Oct4。这些前体细胞以球体形式增殖，能够自我更新，并且可以分化成多种谱系。分化的细胞不仅获得谱系特异性标志物，而且在离体条件下表现出适当的功能。人体皮肤中的大多数Oct 4阳性细胞位于体内细胞角蛋白15染色突出显示的区域。我们的数据提示人胚胎干细胞培养基可用于分离和扩增人成体干细胞。使用这种方法，我们从人类毛囊中分离出一种新的多能成体干细胞群，这些细胞似乎位于凸起区域。人类毛囊可以提供用于治疗应用的成人干细胞的可接近的自体来源。
Hair follicles are known to contain a well-characterized niche for adult stem cells: the bulge, which contains epithelial and melanocytic stem cells. Using human embryonic stem cell culture conditions, we isolated a population of adult stem cells from human hair follicles that are distinctively different from known epithelial or melanocytic stem cells. These cells do not express squamous or melanocytic markers but express neural crest and neuron stem cell markers as well as the embryonic stem cell transcription factors Nanog and Oct4. These precursor cells proliferate as spheres, are capable of self-renewal, and can differentiate into multiple lineages. Differentiated cells not only acquire lineage-specific markers but also demonstrate appropriate functions in ex vivo conditions. most of the Oct4-positive cells in human skin were located in the area highlighted by cytokeratin 15 staining in vivo. our data suggest that human embryonic stem cell medium can be used to isolate and expand human adult stem cells. Using this method, we isolated a novel population of multipotent adult stem cells from human hair follicles, and these cells appear to be located in the bulge area. Human hair follicles may provide an accessible, autologous source of adult stem cells for therapeutic application.