Two new complete mitochondrial genomes of Dorcus stag beetles (Coleoptera, Lucanidae)
Two new complete mitochondrial genomes of Dorcus stag beetles (Coleoptera, Lucanidae)
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两个新的 Dorcus 锹虫（鞘翅目，Lucanidae）线粒体基因组
DOI：
10.1007/s13258-018-0699-8
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发表时间：
2018-08-01
期刊：
影响因子：
2.1
通讯作者：
Wan, Xia
中科院分区：
文献类型：
--
作者：
Chen, YongJing;Liu, Jing;Wan, Xia
Dorcus MacLeay的系统分类一直是一个长期的争论。线粒体基因组因其重要性和全面性而被广泛用于深入研究问题分类群的系统发育。为解决杜父甲系统学争议提供更多有用的遗传学资料。利用下一代测序技术获得了河北Dorcus hopei和Seguyi Dorcus的完整线粒体基因组。通过比较两种基因组的基因组结构和碱基组成、蛋白质编码基因和密码子使用、基因间间隔区和非编码区、转运和核糖体RNA基因以及控制区，阐明了两种基因组的特点。利用最大似然法和贝叶斯推断法对9种鹿角甲虫和3种金龟子的13个PCG序列进行了系统发育关系的重建。对D. hopei和D. seguyi的长度分别为16，026 bp/17，955 bp。D.在A+ T富集区存在一个940 bp的串联重复序列。河北。在D. nad 2和trnW之间发现了一个332 bp的非编码区。seguyi。系统发育分析支持D.河北以D.平行管而D. seguyi不包括分支（D. hopei + D. parallelipipipedus），但他们的姐妹。结果表明，D.河北应该是多克斯·麦克雷的好成员。并对D. seguyi仍有待进一步研究。
The systematics of Dorcus MacLeay has been a long-standing debate. Mitochondrial genomes were widely used to deeply understand the phylogeny of problematic taxa in virtue of their genetic importance and comprehensiveness. To provide more useful genetic data for resolving the systematic disputation of Dorcus stag beetles. The complete mitochondrial genomes of Dorcus hopei and Dorcus seguyi were obtained using the next generation sequencing. Characteristics of the two genomes are explicated through comparing their genome organization and base composition, protein-coding genes and codon usage, intergenic spacers and non-coding region, transfer and ribosomal RNA genes and control region. Phylogenetic relationships were reconstructed using Maximum likelihood and Bayesian inference analyses based on the concatenated nucleotide sequences of 13 PCGs from 9 stag beetles and 3 scarab beetles. The complete mitogenomes of D. hopei and D. seguyi was 16,026 bp/17,955 bp long, respectively. A tandem repeat with the length of 940 bp was presented in the A+T-rich region in D. hopei. An unexpected non-coding region of 332 bp was located between nad2 and trnW in D. seguyi. The phylogenetic analyses robustly supported that D. hopei formed a branch with the generic type of D. parallelipipedus. Whereas D. seguyi was not covered in the branch of (D. hopei + D. parallelipipedus), but was sister to them. The results indicated that D. hopei should be a good member of Dorcus MacLeay. The taxonomic status of D. seguyi remained to be studied furtherly.