Histone H3.1 and H3.3 complexes mediate nucleosome assembly pathways dependent or independent of DNA synthesis
Histone H3.1 and H3.3 complexes mediate nucleosome assembly pathways dependent or independent of DNA synthesis
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DOI：
10.1016/s0092-8674(03)01064-x
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发表时间：
2004-01-09
期刊：
影响因子：
64.5
通讯作者：
Nakatani, Y
中科院分区：
文献类型：
--
作者：
Tagami, H;Ray-Gallet, D;Nakatani, Y
主要组蛋白H3（H3.1）的沉积与DNA复制和可能的DNA修复过程中的DNA合成偶联，而组蛋白变体H3.3作为DNA合成独立沉积途径的替代变体。为了解决历史H3.1和H3.3是如何通过不同的途径沉积到染色质中的，我们已经纯化了这些历史的沉积机制。H3.1和H3.3复合物含有不同的组蛋白伴侣，CAF-1和HIRA，我们表明是必要的，以介导DNA合成依赖和独立的核小体组装，分别。值得注意的是，这些复合物具有H3.1/H3.3和H4中的每一个分子，这表明历史H3和H4作为二聚体单元存在，它们是核小体形成中的重要中间体。这一发现为染色质复制后表观遗传信息的维持提供了新的可能机制。
Deposition of the major histone H3 (H3.1) is coupled to DNA synthesis during DNA replication and possibly DNA repair, whereas histone variant H3.3 serves as the replacement variant for the DNA-synthesis-independent deposition pathway. To address how histories H3.1 and H3.3 are deposited into chromatin through distinct pathways, we have purified deposition machineries for these histories. The H3.1 and H3.3 complexes contain distinct histone chaperones, CAF-1 and HIRA, that we show are necessary to mediate DNA-synthesis-dependent and -independent nucleosome assembly, respectively. Notably, these complexes possess one molecule each of H3.1/H3.3 and H4, suggesting that histories H3 and H4 exist as dimeric units that are important intermediates in nucleosome formation. This finding provides new insights into possible mechanisms for maintenance of epigenetic information after chromatin duplication.