Isolation and characterization of ferrous- and sulfur-oxidizing bacteria from Tengchong solfataric region, China.
Isolation and characterization of ferrous- and sulfur-oxidizing bacteria from Tengchong solfataric region, China.
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DOI：
10.1016/s1001-0742(08)62411-0
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发表时间：
2009
影响因子：
6.9
通讯作者：
C. Jiang;Y. Liu;Yan-yang Liu;Xu Guo;Shuangjiang Liu
中科院分区：
文献类型：
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作者：
C. Jiang;Y. Liu;Yan-yang Liu;Xu Guo;Shuangjiang Liu
微生物对铁、硫的氧化还原作用是酸性环境中土壤地球化学循环的重要组成部分。嗜酸硫杆菌属（Acidithiobacillus）和纤硫杆菌属（Leptocellulum）是这类环境中常见的亚铁和硫氧化菌。本研究的重点是位于中国西南部云南省的腾冲沙发区。通过培养，筛选出9株能在亚铁和硫酸盐上生长的菌株。16 S rRNA基因分析表明，这9株菌分属于嗜酸硫杆菌属（Acidithiobacillus）、脂环酸杆菌属（Alicyclobacillus）、硫杆菌属（Sulfobacillus）、嗜酸细芽孢杆菌属（Leptocellulum）和嗜酸菌属（Acidiphilium）。生理和系统发育研究表明，两株菌株（TC-34和TC-71）可能代表脂环酸芽孢杆菌的两个新成员。菌株TC-34和TC-71与其它脂环酸芽孢杆菌的16 S rRNA基因同源性为94.8%~ 97.1%。TC-34和TC-71菌株与以往报道的脂环酸芽孢杆菌不同，它们是嗜温的，其细胞脂肪酸不含ω-环脂肪酸。菌株TC-71专性依赖亚铁离子生长。结果表明，腾冲盐土区亚铁-铁氧化菌种类多样，脂环酸杆菌可能参与了铁的生物化学地球循环。
Microbial oxidation and reduction of iron and sulfur are important parts of biogeochemical cycles in acidic environments such as geothermal solfataric regions. Species of Acidithiobacillus and Leptospirillum are the common ferrous-iron and sulfur oxidizers from such environments. This study focused on the Tengchong sofataric region, located in Yunnan Province, Southwest China. Based on cultivation, 9 strains that grow on ferrous-iron and sulfuric compounds were obtained. Analysis of 16S rRNA genes of the 9 strains indicated that they were affiliated to Acidithiobacillus, Alicyclobacillus, Sulfobacillus, Leptospirillum and Acidiphilium. Physiological and phylogenetic studies indicated that two strains (TC-34 and TC-71) might represent two novel members of Alicyclobacillus. Strain TC-34 and TC-71 showed 94.8%–97.1% 16S rRNA gene identities to other species of Alicyclobacillus. Different from the previously described Alicyclobacillus species, strains TC-34 and TC-71 were mesophilic and their cellular fatty acids do not contain ω-cyclic fatty acids. Strain TC-71 was obligately dependent on ferrous-iron for growth. It was concluded that the ferrous-iron oxidizers were diversified and Alicyclobacillus species were proposed to take part in biochemical geocycling of iron in the Tengchong solfataric region.