Superficial treatment of mammalian cells using plasma needle
Superficial treatment of mammalian cells using plasma needle
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DOI：
10.1088/0022-3727/36/23/007
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发表时间：
2003-12
期刊：
影响因子：
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通讯作者：
E. Stoffels;I. Kieft;R. Sladek
中科院分区：
文献类型：
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作者：
E. Stoffels;I. Kieft;R. Sladek
研究了在培养中与活细胞中的小型非热等离子体（等离子体针）的相互作用。我们已经证明了血浆针的无损特征：在中等条件下（低功率和低浓度分子物种），血浆针不热生物学样品，也不会诱导细胞死亡。活细胞的处理仅限于细胞外部（膜）。由于血浆自由基与细胞粘附分子的相互作用，细胞附着被暂时中断。可以将松散的细胞去除，重新连接或转移。这种作用在精细的手术中可能非常有用，其中必须以高精度去除组织的一部分，而不会损害相邻细胞，而没有炎症反应。
Interactions of a small-size, non-thermal plasma (plasma needle) with living cells in culture are studied. We have demonstrated the non-destructive character of the plasma needle: under moderate conditions (low-power and low concentration of molecular species) the plasma needle does not heat biological samples and does not induce cell death. Treatment of living cells is restricted to the cell exterior (membrane). As a result of the interactions of plasma radicals with cell adhesion molecules, cell attachment is temporarily interrupted; the loose cells can be removed, reattached or transferred. This effect may prove very useful in fine surgery, where a part of the tissue must be removed with high-precision, without damage to the adjacent cells and without inflammatory reaction.