Characterization of polypropyleneimine as an alternative transfection reagent

Characterization of polypropyleneimine as an alternative transfection reagent
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聚丙烯亚胺作为替代转染试剂的表征

DOI：
10.1007/s44211-023-00284-x
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发表时间：
2022
期刊：
Anal. Sci.
影响因子：
--
通讯作者：
Y. Katayama
Y. Katayama
中科院分区：
--
文献类型：
--
作者：
R. Saeki;S. Kobayashi;R. Shimazui;T. Nii;A. Kishimura;T. Mori;M. Tanaka;Y. Katayama

文献摘要

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将聚丙烯亚胺（PPI）作为转染试剂与目前应用最广泛的聚合物聚乙烯亚胺（PEI）进行了比较。PPI对内体pH的敏感性和对质粒DNA的凝聚能力均优于PEI。虽然PPI的细胞毒性略高于PEI，但在某些细胞系中PPI的转染效率与PEI相当或高于PEI。因此，PPI将是一种替代的转染试剂。
Polypropyleneimine (PPI) was examined as a transfection reagent comparing with most widely used polymer, polyethyleneimine (PEI). PPI had better responsiveness to the endosomal pH and showed more condensation ability of plasmid DNA than PEI. Although the cytotoxicity of PPI was somewhat higher than PEI, the transfection efficacy of PPI was comparable with PEI or higher than PEI in some cell line. Thus, PPI would be an alternative transfection reagent.Graphical abstract