Mild heat stimulation facilitates muscle hypertrophy in C2C12 and mouse satellite cells through myokine release to the culture medium
Mild heat stimulation facilitates muscle hypertrophy in C2C12 and mouse satellite cells through myokine release to the culture medium
复制标题
温和的热刺激通过向培养基释放肌因子促进 C2C12 和小鼠卫星细胞的肌肉肥大
DOI：
10.1016/j.bbrc.2022.10.018
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发表时间：
2022
影响因子：
3.1
通讯作者：
Yonekura Shinichi
中科院分区：
文献类型：
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作者：
Hayashi Satoko;Yonekura Shinichi
温和的热刺激被定义为比最佳细胞培养温度或体温高几度的刺激。据报道，C2C12细胞中的肌肉肥大通过39 °C温和的热刺激而促进。然而，39 °C温和的热刺激促进肌肉肥大的潜在分子机制仍然难以捉摸。在这项研究中，我们的目的是了解这些机制的细节。首先，我们发现39 °C温和的热刺激对C2C12和小鼠原代卫星细胞中的肌生成相关因子（MRF）表达几乎没有影响。因此，我们假设39 °C温和的热刺激通过肌因子（肌生成中的基本组分）促进肌肉肥大。有趣的是，在C2C12和小鼠原代卫星细胞的情况下，我们观察到在37 ° C下在39 °C的条件培养基中培养的组与在37 °C下在37 ° C的条件培养基中培养的组相比肌肉肥大。这些结果表明，39 °C温和的热刺激一旦在培养基中释放，就通过肌因子促进肌肉肥大。最后，我们确定核心蛋白聚糖作为肥大诱导的肌因子候选者。因此，在这项研究中，我们证明了39 °C温和的热刺激通过增强C2C12和小鼠原代卫星细胞中的核心蛋白聚糖基因表达而导致肌肉肥大。
Mild heat stimulation is defined as a stimulation that occurs several degrees above optimal cell culture or body temperatures. Muscle hypertrophy in C2C12 cells is reportedly facilitated by 39 °C mild heat stimulation. However, the underlying molecular mechanisms by which 39 °C mild heat stimulation promotes muscle hypertrophy remains elusive. In this study, we aimed at understanding the details of these mechanisms. First, we found that 39 °C mild heat stimulation has little or no effect on Myogenesis-Related Factor (MRF) expression both in C2C12 and mouse primary satellite cells. Therefore, we hypothesized that 39 °C mild heat stimulation promotes muscle hypertrophy through myokines, essential components in myogenesis. Interestingly, we observed muscle hypertrophy in the group cultured at 37 °C in conditioned medium from 39 °C compared to the group cultured at 37 °C in conditioned medium from 37 °C in the case of both C2C12 and mouse primary satellite cells. These results suggest that 39 °C mild heat stimulation promotes muscle hypertrophy through myokines, once released in the culture medium. Finally, we identified Decorin as a hypertrophy-inducing myokine candidate. Therefore, in this study, we demonstrated that 39 °C mild heat stimulation contributes to muscle hypertrophy through enhancing Decorin gene expression in C2C12 and mouse primary satellite cells.