Essential pathogenic role for endogenous interferon‐gamma (IFN‐γ) during disease onset phase of murine experimental autoimmune orchitis. I. In vivo studies
Essential pathogenic role for endogenous interferon‐gamma (IFN‐γ) during disease onset phase of murine experimental autoimmune orchitis. I. In vivo studies
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内源性干扰素-γ (IFN-γ) 在小鼠实验性自身免疫性睾丸炎发病阶段的重要致病作用 I. 体内研究。
DOI：
10.1046/j.1365-2249.1998.00527.x
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发表时间：
1998
影响因子：
4.6
通讯作者：
Nicoletti
中科院分区：
文献类型：
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作者：
Itoh;Yano;Xie;Iwahashi;Takeuchi;Meroni;Nicoletti
我们以前发现，用同基因睾丸生殖细胞（TGC）免疫CH 3/He雄性小鼠，无需任何佐剂的帮助，足以诱导DTH TGC和实验性自身免疫性睾丸炎（EAO）。为了评估内源性IFN-γ在该模型中的作用，在第0天和第14天皮下接种TGC的C3 H/He小鼠在第15、20或25天接受抗鼠IFN-γ MoAb的单次注射。在第45天，测试DTH至TGC，收集睾丸样本用于组织学检查，并收集血液样本用于IFN-γ测量。结果显示，虽然在第15或25天的MoAb治疗不影响DTH反应、EAO发展和循环中IFN-γ的出现，但在第20天的治疗显著抑制了所有这些反应。因此，只要在疾病发展期间的最佳时间点给予治疗，单次注射抗IFN-γ MoAb就可以成功下调睾丸自身免疫。
We previously found that immunization of CH3/He male mice with syngeneic testicular germ cells (TGC) without the aid of any adjuvants was sufficient to induce DTH to TGC and experimental autoimmune orchitis (EAO). To evaluate the role of endogenous IFN‐γ in this model, C3H/He mice immunized subcutaneously with TGC on days 0 and 14 received a single injection of anti‐murine IFN‐γ MoAb on day 15, 20 or 25. On day 45, DTH to TGC was tested, testis specimens were collected for histological examination, and blood samples collected for IFN‐γ measurement. The results showed that whilst MoAb treatment on day 15 or 25 did not influence DTH responses, EAO development, and appearance of IFN‐γ in the circulation, treatment on day 20 significantly suppressed all of them. Thus, a single injection with anti‐IFN‐γ MoAb may successfully down‐regulate testicular autoimmunity, provided that the treatment is given at an optimal time point during disease development.
鼠白细胞介素 2、γ 干扰素和肿瘤坏死因子对小鼠 Leydig 细胞睾酮合成的直接和间接影响。
影响因子：
--
作者：
Meikle,AW;CardosodeSousa,JC;Dacosta,N;Bishop,DK;Samlowski,WE
通讯作者：
Samlowski,WE
不孕症中的细胞介导的免疫。
DOI：
10.1111/j.1600-0897.1988.tb00197.x
发表时间：
1988
期刊：
American journal of reproductive immunology and microbiology : AJRIM
影响因子：
--
作者：
Anderson,DJ;Hill,JA
通讯作者：
Hill,JA