Ultrasensitive Detection of Aqueous Uranyl Based on Uranyl-Triggered Protein Photocleavage
Ultrasensitive Detection of Aqueous Uranyl Based on Uranyl-Triggered Protein Photocleavage
复制标题
DOI：
10.1002/anie.202115886
复制
发表时间：
2022-01-17
影响因子：
16.6
通讯作者：
Wang, Ning
中科院分区：
文献类型：
--
作者：
Feng, Tiantian;Yuan, Yihui;Wang, Ning
环境铀酰的检测越来越受到人们的关注。然而，现有的检测策略主要依赖于对铀酰的选择性识别，这受到共存金属离子的严重干扰。本文中，基于独特的铀酰触发的光裂解性质，蛋白质BSA用荧光分子标记，所述荧光分子表现出用于铀酰检测的聚集诱导的发射效应。铀酰引发的光裂解导致荧光分子标记的蛋白质片段的分离，导致发射荧光的衰减，其用作铀酰检测的信号。这种检测策略显示出对铀酰的高选择性和24 pM的超低检测限，具有覆盖五个数量级的宽检测范围。该检测方法还显示出高可靠性和稳定性，使其成为在不同环境中实际应用的有前途的技术。
The detection of environmental uranyl is attracting increasing attention. However, the available detection strategies mainly depend on the selective recognition of uranyl, which is subject to severe interference by coexisting metal ions. Herein, based on the unique uranyl-triggered photocleavage property, the protein BSA is labelled with fluorescent molecules that exhibit an aggregation-induced emission effect for uranyl detection. Uranyl-triggered photocleavage causes the separation of the fluorescent-molecule-labelled protein fragments, leading to attenuation of the emission fluorescence, which is used as a signal for uranyl detection. This detection strategy shows high selectivity for uranyl and an ultralow detection limit of 24 pM with a broad detection range covering five orders of magnitude. The detection method also shows high reliability and stability, making it a promising technique for practical applications in diverse environments.