SORTING OF PROTEIN-A TO THE STAPHYLOCOCCAL CELL-WALL
SORTING OF PROTEIN-A TO THE STAPHYLOCOCCAL CELL-WALL
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DOI：
10.1016/0092-8674(92)90101-h
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发表时间：
1992-07-24
期刊：
影响因子：
64.5
通讯作者：
FISCHETTI, VA
中科院分区：
文献类型：
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作者：
SCHNEEWIND, O;MODEL, P;FISCHETTI, VA
革兰氏阳性细菌的细胞壁可以被认为是一个独特的细胞室，其中包含需要特定分选信号的锚定表面蛋白。一些具有重要生物学意义的产物通过这种方式被锚定，包括金黄色葡萄球菌的蛋白A和纤维连接蛋白结合蛋白以及链球菌的M蛋白。对葡萄球菌蛋白A和大肠杆菌碱性磷酸酶的研究表明，细胞壁锚定的必要和充分信号由一个LPXTGX基序、一个C端疏水结构域和一个带电尾巴组成。这些序列元件在不同革兰氏阳性细菌的许多表面蛋白中都是保守的。我们提出了一种迄今为止尚未描述的分选机制，它将蛋白质定位在革兰氏阳性细菌的表面。
The cell wall of gram-positive bacteria can be thought of as representing a unique cell compartment, which contains anchored surface proteins that require specific sorting signals. Some biologically important products are anchored in this way, including protein A and fibronectin binding protein of Staphylococcus aureus and streptococcal M protein. Studies of staphylococcal protein A and Escherichia coli alkaline phosphatase show that the signal both necessary and sufficient for cell wall anchoring consists of an LPXTGX motif, a C-terminal hydrophobic domain, and a charged tail. These sequence elements are conserved in many surface proteins from different gram-positive bacteria. We propose the existence of a hitherto undescribed sorting mechanism that positions proteins on the surface of gram-positive bacteria.