Optically Driven Micro-pinch to Mechanically Analyze Cellular Protein
Optically Driven Micro-pinch to Mechanically Analyze Cellular Protein
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光学驱动微捏对细胞蛋白质进行机械分析
DOI：
10.1016/j.procir.2013.01.025
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发表时间：
2013
期刊：
影响因子：
--
通讯作者：
K Ikuta
中科院分区：
文献类型：
--
作者：
N Shimada;K Kadoguchi;M Ikeuchi;K Ikuta
我们研制了一种新的分析细胞蛋白质力学性质的装置。为了测量细胞骨架蛋白质的机械性质，传统上利用通过光镊效应的微珠操纵方法。然而，由于微球的形状仅为球形，这种方法存在两个问题：（1）对实验类型的限制，（2）用于光镊的强激光靠近目标蛋白。这些都是分子生物学中不可缺少的缺点，如细胞内和细胞外蛋白质的测量。在目前的工作中，我们已经创建了一个新的工具，取代微珠命名为“光学驱动微箍缩”的测量极小的蛋白质的机械性能。微夹有两个用于夹蛋白质的小板。使用微箍缩，关于蛋白质的机械分析的各种实验成为可能。
We developed a new device for analyzing mechanical properties of cellular proteins. To measure mechanical properties of cytoskeleton proteins, micro-bead manipulating method by optical tweezers effect has conventionally been utilized. However, such method had two problems since micro-bead shape is only sphere: (1) restrictions on the type of experiments, and (2) strong laser for optical tweezers is close to the target proteins. These were indispensable disadvantages in molecular biology such as measurement of intracellular and extracellular proteins. In present work, we have created a novel tool taking over micro-bead named “Optically Driven Micro-Pinch” for measuring mechanical properties of extremely small proteins. The micro-pinch has two small plates for pinching the proteins. Using the micro-pinch, various experiments about mechanical analysis of the proteins becomes possible.