Preparation of molecularly imprinted hybrid monoliths for the selective detection of fluoroquinolones in infant formula powders
Preparation of molecularly imprinted hybrid monoliths for the selective detection of fluoroquinolones in infant formula powders
复制标题
用于选择性检测婴儿配方奶粉中氟喹诺酮类药物的分子印迹杂化整体材料的制备
DOI：
10.1016/j.chroma.2018.12.038
复制
发表时间：
2019-03-15
影响因子：
4.1
通讯作者：
Wang, Qiqin
中科院分区：
文献类型：
--
作者：
Shao, Huikai;Zhou, Haibo;Wang, Qiqin
以左氧氟沙星（LEV）为模板分子，3-氨丙基三乙氧基硅烷-甲基丙烯酸为杂化功能单体，乙二醇二甲基丙烯酸酯为交联剂，在甲醇、甲苯和十二醇的混合致孔剂中，采用一步聚合法制备了一种新型的分子印迹无机-有机杂化整体柱（MIP杂化整体柱）。采用扫描电子显微镜和傅立叶变换红外光谱对优化后的LEV-MIP杂化整体柱进行了表征。结果表明，LEV-MIP杂化整体柱的网络骨架中具有均匀的整体骨架和较大的通孔。系统地研究了聚合条件对整体柱特异性识别行为的影响。与未印迹的杂化整体柱相比，LEV-MIP杂化整体柱对LEV具有更好的吸附性能（3.62倍）和选择性。将LEV-MIP杂化整体柱固相萃取-液相色谱-质谱联用技术应用于婴幼儿配方粉中氟喹诺酮类药物的选择性检测。在20、50和100 μ g kg（-1）的添加水平下，6种氟喹诺酮类药物的平均回收率为82.91-102.00%，精密度为1.04- 7.39%。该方法的检出限和定量限分别为0.19-1.24 μ g kg（-1）和0.63-4.13 μ g kg（-1）。(C)2018爱思唯尔B. V.保留所有权利。
A novel molecularly imprinted inorganic-organic hybrid monolith (MIP hybrid monolith) was fabricated through a facile single-step polymerization strategy with levofloxacin (LEV) as the template, 3-aminopropyltriethoxysilane-methacrylic acid as the hybrid functional monomer and ethylene glycol dimethacrylate as the crosslinker in a mixed porogen of methanol, toluene and dodecanol. The optimized LEV-MIP hybrid monolith was characterized using scanning electron microscopy and fourier transform-infrared spectroscopy. Uniform monolithic matrix with large through-pores in the network skeleton of LEV-MIP hybrid monolith was observed. The influence of polymerization conditions on the specific recognition behavior of the resulting monolith was systematically investigated. The LEV-MIP hybrid monolith exhibited much better adsorption (3.62 times) and selectivity towards LEV in comparison with non imprinted hybrid monolith. Furthermore, the LEV-MIP hybrid monolith based solid-phase extraction combining with liquid chromatography-mass spectrometry was applied for the selective determination of fluoroquinolones (FQs) in infant formula powder. The average recoveries of six FQs in milk powders spiked at 20, 50 and 100 mu g kg(-1) were in the range of 82.91-102.00% with the precision of 1.04-7.39%. The limit of detection and limit of quantitation of the proposed method were in a range of 0.19-1.24 mu g kg(-1) and 0.63-4.13 mu g kg(-1), respectively. (C) 2018 Elsevier B.V. All rights reserved.