Induction and isolation of artificial pigmentation mutants in Porphyra haitanensis Chang et Zheng (Bangiales, Rhodophyta)
Induction and isolation of artificial pigmentation mutants in Porphyra haitanensis Chang et Zheng (Bangiales, Rhodophyta)
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发表时间：
2005
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通讯作者：
Aruga Yusho
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作者：
Aruga Yusho
坛紫菜幼配子体叶片由野生型的壳孢子发育而来，用 60Co-γ射线处理诱导突变。培养1周后，处理后的叶片中出现紫红色、粉红色、红橙色、橙色、黄绿色、绿色等突变颜色的颜色突变细胞。培养3周后，颜色突变的细胞长成不同颜色的细胞团。当照射剂量从0 Gy增加到1100 Gy时，颜色突变细胞簇的频率随着剂量的增加而增加。然而，进一步增加剂量至 1400 Gy，颜色突变细胞簇的频率降低。这些结果表明，1100Gy是诱发坛松叶片突变的合适剂量。叶片不同部位颜色突变细胞簇的频率不同，从叶片基部到尖端逐渐增加。从颜色突变的叶片中酶解分离单细胞，并再生为叶片，其中出现一些单色突变叶片和一些由颜色突变细胞和野生型细胞组成的斑点杂色叶片。通过单性生殖从单色突变体叶片中建立了5个颜色为红棕色、深橙色、粉红色、金棕色和暗黄绿色的色素沉着突变株(conchocelis)。它们分别被命名为 tan、bor、ceb、hon 和 cac。它们的颜色在配子体叶片和丝状体阶段都相对稳定。各突变株的F1配子体叶片分别与其母体叶片呈现相同的颜色。
The young gametophytic blades of Porphyra haitanensis Chang et Zheng, developed from the conchospores of the wild type, were treated with 60Co-γ ray to induce mutations. Color-mutated cells showing purplish red, pink, red orange, orange, yellow green, green and other mutated colors appeared in the treated blades after culture for 1 week. After culture of 3 weeks, the color-mutated cells grew to be cell-clusters with different colors. When the irradiation dose increased from 0 Gy to 1100 Gy, the frequency of the color-mutated cell-clusters was higher with increase of the dose. However, further increase of the dose up to 1400 Gy, decreased the frequency of the color-mutated cell-clusters. These results indicated that 1100 Gy is the suitable dose for inducing mutations of P. haitanensis blades. The frequency of the color-mutated cell-clusters was different in the different parts of a blade, increasing from the base to the tip of the blade. Single cells were isolated enzymatically from the color-mutated blades, and regenerated into blades among where appeared some single-colored mutant blades and a few spottedly variegated blades consisting color-mutated cells and the wild-type cells. Five pigmentation mutant strains (conchocelis) with colors of red brown, dark orange, pink, golden brown and dull yellow green were established from the single-colored mutant blades by parthenogenesis. They were named as tan, bor,ceb, hon and cac, respectively. Their colors were relatively stable in both gametophytic blade and conchocelis phases. The F1 gametophytic blades of each mutant strains, showed the same color with its mother blades, respectively.